{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:13:45Z","timestamp":1788426825310,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32560721"],"award-info":[{"award-number":["32560721"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160700"],"award-info":[{"award-number":["32160700"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    bHLH transcription factors serve as crucial regulators of plant growth, development, and responses to abiotic stress. However, their specific functions in the cold resistance of sweet cherry remain elusive. In this study, we cloned\n                    <jats:italic>PavbHLH162\u2010like<\/jats:italic>\n                    and performed gain\u2010 and loss\u2010of\u2010function assays to explore its role under cold stress.\n                    <jats:italic>PavbHLH162\u2010like<\/jats:italic>\n                    was upregulated by cold stress, and its encoded protein was localized in the nucleus and possessed transcriptional activation activity. Overexpression of\n                    <jats:italic>PavbHLH162\u2010like<\/jats:italic>\n                    in tobacco significantly enhanced cold tolerance, as evidenced by reduced malondialdehyde (MDA) content and reactive oxygen species (ROS) accumulation, along with increased levels of soluble sugars, soluble proteins, and antioxidant enzyme activities compared to the wild\u2010type (WT) plants. Conversely, silencing\n                    <jats:italic>PavbHLH162\u2010like<\/jats:italic>\n                    increased cold sensitivity, leading to elevated MDA and ROS levels, and decreased contents of soluble compounds and antioxidant enzyme activities. Additionally, overexpression of\n                    <jats:italic>PavbHLH162\u2010like<\/jats:italic>\n                    enhances seed germination and root growth in tobacco. Both overexpression and silencing affected the expression of cold\u2010related genes. Yeast two\u2010hybrid and luciferase assays demonstrated that PavbHLH162\u2010like interacts with PavbHLH106. Furthermore, PavbHLH162\u2010like binds to the promoter of\n                    <jats:italic>PavNAC29\u2010like<\/jats:italic>\n                    and activates its transcription. This activation was strengthened by the PavbHLH162\u2010like\u2010PavbHLH106 complex. Collectively, our findings indicate that\n                    <jats:italic>PavbHLH162\u2010like<\/jats:italic>\n                    acts as a positive regulator of cold tolerance in sweet cherry, which provides insights into the molecular mechanisms of cold resistance in this species.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71042","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:04:48Z","timestamp":1785557088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>PavbHLH162<\/scp>\n                    \u2010Like\u2010\n                    <scp>PavbHLH106<\/scp>\n                    Module Positively Regulates Cold Tolerance in Sweet Cherry (\n                    <scp>\n                      <i>Prunus avium<\/i>\n                    <\/scp>\n                    L.)"],"prefix":"10.1111","volume":"178","author":[{"given":"Jin","family":"Tang","sequence":"first","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences\/Institute of Agro\u2010Bioengineering Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiandong","family":"Hou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences\/Institute of Agro\u2010Bioengineering Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulin","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences\/Institute of Agro\u2010Bioengineering Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixue","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences\/Institute of Agro\u2010Bioengineering Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2673-3375","authenticated-orcid":false,"given":"Yuxin","family":"Leng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences\/Institute of Agro\u2010Bioengineering Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Gu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant 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