{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:13:42Z","timestamp":1788426822693,"version":"build-2803163510"},"reference-count":68,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2026MS0288"],"award-info":[{"award-number":["ZR2026MS0288"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Broccoli harvest involves cutting the stalk and creating a wound site known as the incision site (IC), which immediately triggers a self\u2010adaptive stage (SAS, ~12\u2009h) before precooling. This early postharvest stage is critical for wound perception, signaling, healing, redox balance, and mineral redistribution. Here, we employed a multi\u2010omics approach combining transcriptomics, metabolomics, and mineralomics to characterize dynamic responses at the IC across 0, 3, 6, and 12\u2009h post\u2010wounding. A total of 6599 shared DEGs were identified across timepoints, with 4137 DEGs specific to 3 versus 0\u2009h, substantially more than at 6 or 12\u2009h. GO enrichment analysis revealed rapid induction of abiotic stress responses, transmembrane transport, and kinase signaling. Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2013ROS\u2013MAPK signaling genes (\n                    <jats:italic>ATP2C<\/jats:italic>\n                    ,\n                    <jats:italic>CML<\/jats:italic>\n                    ,\n                    <jats:italic>CPK<\/jats:italic>\n                    ,\n                    <jats:italic>MPK4<\/jats:italic>\n                    ,\n                    <jats:italic>RBOH<\/jats:italic>\n                    ) were rapidly upregulated at 3\u2009h, along with hormone\u2010related genes:\n                    <jats:italic>LOX2S<\/jats:italic>\n                    ,\n                    <jats:italic>AOC<\/jats:italic>\n                    ,\n                    <jats:italic>OPR<\/jats:italic>\n                    ,\n                    <jats:italic>AOS<\/jats:italic>\n                    (jasmonic acid),\n                    <jats:italic>ABAI<\/jats:italic>\n                    ,\n                    <jats:italic>NCED<\/jats:italic>\n                    (abscisic acid), and\n                    <jats:italic>ACS<\/jats:italic>\n                    (ethylene). Wound healing genes, for example,\n                    <jats:italic>PAL<\/jats:italic>\n                    ,\n                    <jats:italic>4CL<\/jats:italic>\n                    , and redox\u2010related genes like\n                    <jats:italic>GST<\/jats:italic>\n                    and\n                    <jats:italic>GPX<\/jats:italic>\n                    , exhibited maximal expression at 3\u2009h. Mineralomics revealed transient surges in Fe, K\n                    <jats:sup>+<\/jats:sup>\n                    , and Na\n                    <jats:sup>+<\/jats:sup>\n                    at 3\u2009h, tightly correlated with upregulation of ion transporters (\n                    <jats:italic>HKT<\/jats:italic>\n                    ,\n                    <jats:italic>SLC47A<\/jats:italic>\n                    family). Moreover, metabolomics showed the accumulation of signaling lipids, hormone derivatives, phenylpropanoids, and sulforaphane at 3\u2009h, confirming the first 3\u2009h as the primary metabolic reprogramming window. These findings suggest that interventions to enhance wound repair, redox stability, and mineral homeostasis should be taken within 3\u2009h to delay senescence and extend broccoli postharvest shelf life.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71046","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T04:52:26Z","timestamp":1786337546000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Holistic Omics\u2010Based Overview of Postharvest Wound Response and Mineral Dynamics in Broccoli Stalks During the Self\u2010Adaptive Stage"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9174-9700","authenticated-orcid":false,"given":"Aqleem","family":"Abbas","sequence":"first","affiliation":[{"name":"College of Agricultural Engineering and Food Science Shandong University of Technology  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni Heng","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Agricultural Engineering and Food Science Shandong University of Technology  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3182-7487","authenticated-orcid":false,"given":"Yanyin","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Agricultural Engineering and Food Science Shandong University of Technology  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9761-7073","authenticated-orcid":false,"given":"Muhammad","family":"Asim","sequence":"additional","affiliation":[{"name":"College of Agricultural Engineering and Food Science Shandong University of Technology  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingkun","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Agricultural Engineering and Food Science Shandong University of Technology  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2025.114058"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2005.12.006"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000675107"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2025.103921"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2024.113183"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-024-04918-8"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694-023-01841-8"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694-024-02415-y"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.343.6168.290"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0317-7"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2024.113261"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(00)00135-7"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/f13081258"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2020.111363"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1221294110"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.33320\/maced.pharm.bull.2015.61.01.007"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.061432"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24193537"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2018.07.003"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16845"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2017.06.008"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085524"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19574-w"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08608"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3496"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0733-5_5"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.072686"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-010-1269-8"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12984"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2016.09.009"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-025-11409-z"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.4141\/P99-014"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44319-025-00471-z"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16106"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2012.09.013"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2008.10.001"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2005.05.009"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2011.02.029"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.107544"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11947-023-03275-y"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep44729"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-015-9829-9"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40003-021-00594-4"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.114.244871"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-007-9164-9"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00470"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202153817"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2023.112669"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.02.029"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12393"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.17660\/ActaHortic.2013.1005.10"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2018.07.010"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat7744"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13562-020-00621-5"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-369-5_2"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1538"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10725-018-0464-7"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.068809"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2022.113529"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes11120984"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.110434"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae573"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-211"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.13315"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.71046","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ppl.71046","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.71046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T08:22:21Z","timestamp":1788423741000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.71046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":68,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/ppl.71046"],"URL":"https:\/\/doi.org\/10.1111\/ppl.71046","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2025-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71046"}}