{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:51:40Z","timestamp":1789487500413,"version":"build-2803163510"},"reference-count":79,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Soybean (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Glycine max<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) seed germination is highly sensitive to saline\u2010alkaline stress. Seed priming represents an effective strategy to mitigate its detrimental effects. However, the optimal priming conditions (agent, concentration, duration) and the underlying molecular mechanisms remain poorly understood. This study investigated the effects of priming with distilled water (Control), calcium chloride (CaCl\n                    <jats:sub>2<\/jats:sub>\n                    ), melatonin (MT), and proline (Pro) under saline\u2010alkaline stress on soybean seed germination and the molecular basis of enhanced tolerance. Evaluation of ten germination\u2010related parameters revealed that priming with 100\u2009mM CaCl\n                    <jats:sub>2<\/jats:sub>\n                    for 12\u2009h significantly enhanced the germination rate. Physiological analyses demonstrated that CaCl\n                    <jats:sub>2<\/jats:sub>\n                    priming effectively reduced reactive oxygen species (ROS) accumulation by increasing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), while decreasing malondialdehyde (MDA) content. Furthermore, CaCl\n                    <jats:sub>2<\/jats:sub>\n                    priming activated the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling pathway by increasing radicle Ca\n                    <jats:sup>2+<\/jats:sup>\n                    content and upregulating the expression levels of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling\u2010related genes (e.g.,\n                    <jats:italic>GmCAM7<\/jats:italic>\n                    ,\n                    <jats:italic>GmCNGC2<\/jats:italic>\n                    ,\n                    <jats:italic>GmCNGC19<\/jats:italic>\n                    ,\n                    <jats:italic>GmMPK2<\/jats:italic>\n                    , and\n                    <jats:italic>GmMKK2<\/jats:italic>\n                    ). Additionally, CaCl\n                    <jats:sub>2<\/jats:sub>\n                    priming significantly enhanced DNA damage repair capacity of soybean cultivars with differing saline\u2010alkaline tolerance. This was manifested by reduced DNA oxidative damage and decreased random amplified polymorphic DNA (RAPD) polymorphism, thereby enhancing genomic stability and alleviating cell cycle arrest. These findings deepen our understanding of the complex regulatory role of calcium signaling in plant abiotic stress responses and provide important novel theoretical insights for improving crop resilience.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71055","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:24:48Z","timestamp":1789025088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>\n                      CaCl\n                      <sub>2<\/sub>\n                    <\/scp>\n                    Priming Boosts Salinity\u2010Alkalinity Tolerance in Germinating Soybean by Reducing\n                    <scp>DNA<\/scp>\n                    Oxidative Damage and Enhancing Ca\n                    <sup>2+<\/sup>\n                    \u2010\n                    <scp>ROS<\/scp>\n                    Signaling Crosstalk"],"prefix":"10.1111","volume":"178","author":[{"given":"Ran","family":"Sun","sequence":"first","affiliation":[{"name":"College of Agriculture Heilongjiang Bayi Agricultural University  Daqing China"},{"name":"Crop Germplasm Resource Innovation Laboratory, National Coarse Cereals Engineering Research Center  Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Agriculture Heilongjiang Bayi Agricultural University  Daqing China"},{"name":"Crop Germplasm Resource Innovation Laboratory, National Coarse Cereals Engineering Research Center  Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture Heilongjiang Bayi Agricultural University  Daqing China"},{"name":"Crop Germplasm Resource Innovation Laboratory, National Coarse Cereals Engineering Research Center  Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Agriculture Heilongjiang Bayi Agricultural University  Daqing China"},{"name":"Crop Germplasm Resource Innovation Laboratory, National Coarse Cereals Engineering Research Center  Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjie","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture Heilongjiang Bayi Agricultural University  Daqing China"},{"name":"Crop Germplasm Resource Innovation Laboratory, National Coarse Cereals Engineering Research Center  Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agriculture Heilongjiang Bayi Agricultural University  Daqing China"},{"name":"Crop Germplasm Resource Innovation Laboratory, National Coarse Cereals Engineering 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