{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:13:33Z","timestamp":1788426813357,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The resin of masson pine (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pinus massoniana<\/jats:italic>\n                    <\/jats:styled-content>\n                    L.) exhibits considerable economic value and primarily consists of diterpenoid resin acids. However, the upstream regulation of the key diterpene biosynthetic gene,\n                    <jats:italic>PmLAS<\/jats:italic>\n                    , remains largely unclear. In the present study, through yeast one\u2010hybrid screening, three PmMYB transcription factors were identified: PmMYB30, PmMYB68, and PmMYB74. Subsequent verification through dual\u2010luciferase assays substantiated that these transcription factors interact with the\n                    <jats:italic>PmLAS<\/jats:italic>\n                    promoter, thereby facilitating its transcriptional activation. Subcellular localization analysis indicated that all three PmMYBs are localized in the nucleus, and transcriptional activation assays in yeast further revealed that their C\u2010terminal regions are responsible for the transcriptional activation activity. Meanwhile, the transcript levels of three\n                    <jats:italic>PmMYBs<\/jats:italic>\n                    exhibited a significant positive correlation with resin yield in the xylem of 12\u2010year\u2010old masson pine trees. Moreover, the heterologous overexpression of these genes in tobacco resulted in a growth\u2010inhibiting phenotype, a marked increase in leaf glandular trichome numbers, and an elevated proportion of diterpenoids compared to wild\u2010type tobacco. To further elucidate the molecular regulation of the\n                    <jats:italic>PmLAS<\/jats:italic>\n                    gene by the transcription factor PmMYB30, yeast two\u2010hybrid library screening was performed to screen its interacting proteins. A RING\u2010type E3 ubiquitin ligase was identified from the screening results and designated PmRDUF1. Subsequent luciferase complementation imaging assays provided further evidence supporting the interaction between PmRDUF1 and PmMYB30. Furthermore, dual\u2010luciferase reporter assays demonstrated that this interaction suppresses the ability of PmMYB30 to activate the expression of\n                    <jats:italic>PmLAS<\/jats:italic>\n                    , forming a PmRDUF1\u2010PmMYB30\u2010PmLAS regulatory module. Collectively, our findings suggest that a complex transcriptional network governs diterpenoid biosynthesis in masson pine, offering new perspectives on the regulation of terpenoid metabolism.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71059","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T07:53:12Z","timestamp":1786089192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>PmRDUF1<\/scp>\n                    \u2010\n                    <scp>PmMYB30<\/scp>\n                    \u2010\n                    <scp>PmLAS<\/scp>\n                    Regulatory Module Is Involved in Terpenoid Biosynthesis of\n                    <scp>\n                      <i>Pinus massoniana<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"178","author":[{"given":"Luonan","family":"Shen","sequence":"first","affiliation":[{"name":"Institute for 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Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering\/College of Life Science Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Min","sequence":"additional","affiliation":[{"name":"Institute for Forest Resources &amp; Environment of Guizhou\/College of Forestry Guizhou University  Guiyang Guizhou Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Peng","sequence":"additional","affiliation":[{"name":"Institute for Forest Resources &amp; Environment of Guizhou\/College of Forestry Guizhou University  Guiyang Guizhou Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaojin","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering\/College of Life Science Guizhou 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