{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:14:02Z","timestamp":1788426842293,"version":"build-2803163510"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":50,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    is a devastating pathogen in viticulture, causing gray mold, and bunch rot. Under 25\u00b0C, 90% relative humidity and 36\u2009h wetness duration,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    can lead to yield losses ranging from 20% to 50% in grapevines. This study evaluated the antifungal effects of\n                    <jats:italic>Trichoderma afroharzianum<\/jats:italic>\n                    secondary metabolite filtrates (TSMF) against the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolate MBc5. In\u00a0vitro dual culture assays revealed that 10\n                    <jats:italic>T. afroharzianum<\/jats:italic>\n                    isolates inhibited the colony growth of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    by 42%\u201388%. TSMF were collected after 30\u2009days of incubation in yeast peptone glucose (YPG) medium and incorporated into potato dextrose agar (PDA) at 10% (v\/v) for inhibition testing against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    . The filtrates significantly inhibited\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    colony growth by 72%\u201392%. Based on these results, three highly effective isolates (Tr28, Tr138, and Tr153) were selected for in\u00a0vivo assays. Leaves and bunches were inoculated with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    (1\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    conidia mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and treated, 2\u2009h later with TSMF (2\u2009mL each). TSMF of Tr153 significantly reduced disease severity in leaves (52%\u201378%) and bunches (70.4%) compared to the pathogen control. Gas chromatography\u2013mass spectrometry (GC\u2013MS) analysis identified butyl acetate as the main component, constituting 39.08% of the Tr153 secondary metabolite filtrate. These results demonstrate that TSMF from\n                    <jats:italic>T. afroharzianum<\/jats:italic>\n                    has strong potential as a biological control agent against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    in grapevines.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71073","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T03:17:53Z","timestamp":1787282273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biocontrol of\n                    <i>Vitis vinifera<\/i>\n                    Gray Mold via a Culture Filtrate of\n                    <i>Trichoderma afroharzianum<\/i>"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5859-9026","authenticated-orcid":false,"given":"Yunus","family":"Korkom","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, Department of Plant Protection Ayd\u0131n Adnan Menderes University  Ayd\u0131n T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/18.2.265a"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1154370"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.9.2380"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO\u201010\u201014\u20100264\u2010R"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01081"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010024\u201002626\u20104"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2024.101530"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364\u20103703.2011.00783.x"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41938\u2010021\u201000488\u20101"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205\u2010020\u201002324\u2010z"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2022.7215"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2025.108299"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529\u2010024\u201003498\u20109"},{"key":"e_1_2_12_15_1","unstructured":"FAO.2024.\u201cFAO.\u201dhttps:\/\/www.fao.org\/faostat\/en\/#data\/QCL."},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12020237"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2022.105019"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2019.02.009"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8100989"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c04540"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b00710"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41938\u2010021\u201000423\u20104"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof10110755"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/applmicrobiol4020060"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095\u20103119(20)63415\u20103"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.5424\/sjar\/2018161-11378"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajgw.12545"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2025.111203"},{"key":"e_1_2_12_30_1","unstructured":"Korkom Y.2022.\u201cDetermination of the Effects ofTrichodermaspp. Obtained From Agricultural Areas in Aydin Province on Charcoal Rot Disease in Strawberry and Plant Growth.\u201dMaster's thesis Ayd\u0131n Adnan Menderes University Ayd\u0131n Turkey."},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B0860015"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026092"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1493272"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.17957\/IJAB\/15.1968"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024222206991"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9091962"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12385"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12091271"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2022.12.002"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.22034\/jchr.2023.1960279.1563"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2015.07.010"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.9755\/ejfa.2020.v32.i12.2222"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201052301\u20102"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1061112"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy10111822"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2023.105424"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e29031"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.21767\/2248\u20109215.100053"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11010081"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340\u2010025\u201001980\u20105"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1556\/038.2025.00243"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/tqem.22071"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003578406-7"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-16503-0_14"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-6040-4_2"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658\u2010015\u20100698\u20104"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10123\u2010019\u201000089\u2010x"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.604923"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.2000.00887.x"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10526\u2010025\u201010379\u20101"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.202400569"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472\u2010765X.2006.01939.x"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6728"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201070975\u20106"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/jse.12447"},{"issue":"1","key":"e_1_2_12_66_1","first-page":"315","article-title":"Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics","volume":"18","author":"White T.","year":"1990","journal-title":"PCR Protocols: A Guide to Methods and Applications"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2024.102395"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118958308.ch8"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00848"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.3852\/13\u2010032"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.71073","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ppl.71073","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.71073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T08:24:23Z","timestamp":1788423863000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.71073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":69,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/ppl.71073"],"URL":"https:\/\/doi.org\/10.1111\/ppl.71073","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71073"}}