{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:14:03Z","timestamp":1788426843122,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":52,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1201600"],"award-info":[{"award-number":["2022YFD1201600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Coptis chinensis<\/jats:italic>\n                    Franch. is a perennial and valuable medicinal plant. The dried rhizome of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. chinensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    has been extensively used in traditional Chinese medicine to treat heat\u2010related disorders. Benzylisoquinoline alkaloids (BIAs), including berberine, epiberberine, palmatine, and coptisine, constitute the primary bioactive compounds in this species. Although light quality is recognized as a key regulator of plant growth and metabolism, the specific roles of distinct light spectra in modulating BIA biosynthesis remain poorly understood. In this study, an integrated transcriptomics and metabolomics analysis was conducted to uncover the regulatory mechanisms underlying light quality\u2010mediated BIA production. Results revealed that red light stimulated plant growth, whereas ultraviolet light inhibited it; however, both light qualities significantly enhanced BIA accumulation. We identified 29 candidate transcription factors (TFs) critical for regulating BIA biosynthesis. Subsequent dual\u2010luciferase reporter assays indicated that\n                    <jats:italic>CcWRKY41<\/jats:italic>\n                    and\n                    <jats:italic>CcWRKY70<\/jats:italic>\n                    could activate several functional genes (\n                    <jats:italic>CcCNMT<\/jats:italic>\n                    ,\n                    <jats:italic>CcOMT1<\/jats:italic>\n                    , and\n                    <jats:italic>CcOMT2<\/jats:italic>\n                    ) in the BIA biosynthetic pathway. Further yeast one\u2010hybrid assays revealed that these TFs directly bound to the\n                    <jats:italic>CcOMT1<\/jats:italic>\n                    promoter, and their transient overexpression significantly elevated BIA levels in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. chinensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . This study demonstrates that\n                    <jats:italic>CcWRKY41<\/jats:italic>\n                    and\n                    <jats:italic>CcWRKY70<\/jats:italic>\n                    are key regulators of light\u2010quality\u2010mediated BIA biosynthesis. These findings provide novel insights into precision agriculture strategies aimed at enhancing the production of high\u2010value BIAs in medicinal plants.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71074","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T08:01:24Z","timestamp":1787385684000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Regulatory Mechanisms of Benzylisoquinoline Alkaloid Biosynthesis in\n                    <i>Coptis chinensis<\/i>\n                    Under Light Quality Treatment"],"prefix":"10.1111","volume":"178","author":[{"given":"Yushu","family":"Xie","sequence":"first","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruikang","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanjia","family":"Nie","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Yu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kedi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuegang","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7339-9865","authenticated-orcid":false,"given":"Yingchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Coptis Development and Utilization (Ministry of Education) College of Pharmaceutical Sciences, Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1377318"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nexres.2025.100320"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010021\u201000559\u20102"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2025.107742"},{"key":"e_1_2_11_6_1","first-page":"114","article-title":"Effects of Different Light Spectra on Alkaloid Contents in Coptis chinensis","volume":"26","author":"Chen J.","year":"2011","journal-title":"Journal of Northwest Forestry University"},{"key":"e_1_2_11_7_1","first-page":"326","volume-title":"Pharmacopoeia of the People's Republic of China. 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