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The present study established an efficient regeneration and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010mediated transformation system for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. sibirica<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Stem segments served as explants, and the optimal proliferation medium was Murashige and Skoog medium supplemented with 0.8\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    6\u2010benzylaminopurine (6\u2010BA), 0.2\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    thidiazuron (TDZ), and 0.1\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    1\u2010naphthaleneacetic acid (NAA), resulting in a proliferation coefficient of 6.53. The genetic transformation system utilized\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. tumefaciens<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain GV3101 carrying reporter vectors pRI101\u2010GFP or pCAMBIA1301\u2010GUS, with optimal parameters including a 3\u2010day preculture, infection at OD\n                    <jats:sub>600<\/jats:sub>\n                    \u2009=\u20090.6 for 20\u2009min, and a 2\u2010day coculture. Transformants were selected in a medium containing 20\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    kanamycin and 300\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    cefotaxime, achieving an average transformation efficiency of 11.27%. As an initial application,\n                    <jats:italic>PsWRKY17<\/jats:italic>\n                    \u2010overexpressing lines were generated, exhibiting transcript levels 7\u201318 times higher than those in the empty\u2010vector control. Following low\u2010temperature treatment,\n                    <jats:italic>PsWRKY17<\/jats:italic>\n                    \u2010OE lines demonstrated increased SOD and POD activities and reduced relative electrical conductivity and MDA content compared to the control (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). These results provide preliminary physiological evidence that\n                    <jats:italic>PsWRKY17<\/jats:italic>\n                    overexpression is associated with altered low\u2010temperature stress responses in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. sibirica<\/jats:italic>\n                    <\/jats:styled-content>\n                    . However, the regulatory mechanism of\n                    <jats:italic>PsWRKY17<\/jats:italic>\n                    requires further investigation. In summary, an effective regeneration and genetic transformation system for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. sibirica<\/jats:italic>\n                    <\/jats:styled-content>\n                    was developed, providing a foundation for future research and biotechnological applications.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71080","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:55:04Z","timestamp":1787061304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of an\n                    <i>Agrobacterium<\/i>\n                    \u2010Mediated Genetic Transformation System for\n                    <i>Prunus sibirica<\/i>\n                    and Its Preliminary Application in\n                    <scp>\n                      <i>PsWRKY17<\/i>\n                    <\/scp>\n     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