{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:15:00Z","timestamp":1788347700210,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["PL2024C036"],"award-info":[{"award-number":["PL2024C036"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32573152"],"award-info":[{"award-number":["32573152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Glucosinolates in two genotypes of kale sprouts treated with methyl jasmonate (MeJA) were analyzed using ultrahigh\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight mass spectrometry (UHPLC\u2010QTOF\u2010MS). This approach enabled the identification of 13 distinct glucosinolates (GSLs) and the quantitative determination of their individual contents. Our results show that MeJA induced GSL biosynthesis, resulting in a significant increase in the total content of aliphatic, indolic, and aromatic GSLs, while also altering their compositional profiles. Notably, the contents of sinigrin (SIN), glucoraphanin (GRA), glucobrassicin (GBC), and glucotropaeolin (GTL) were significantly increased, accompanied by upregulation of key biosynthetic genes, including\n                    <jats:italic>BoMYB51<\/jats:italic>\n                    ,\n                    <jats:italic>BoMYB122<\/jats:italic>\n                    ,\n                    <jats:italic>BoSOT18<\/jats:italic>\n                    ,\n                    <jats:italic>BoST5a<\/jats:italic>\n                    , and\n                    <jats:italic>BoCYP79A2<\/jats:italic>\n                    . MeJA also promoted the accumulation of total phenolics and flavonoids and enhanced CAT and POD activities, associated with improved DPPH radical scavenging activity. However, these metabolic changes were associated with significant growth inhibition, suggesting a trade\u2010off between phytochemical enhancement and biomass production. Genotype\u2010dependent differences were observed between the two lines, with R6 showing stronger anthocyanin induction but weaker GSL responses than W3, indicating that pre\u2010existing metabolic background determines MeJA efficacy. These findings confirm the elicitor effects of MeJA while highlighting the need to balance growth and nutritional quality in potential applications.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71094","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:33:22Z","timestamp":1788345202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exogenous Methyl Jasmonate Promotes Glucosinolate Accumulation and Antioxidant Capacity in Kale Sprouts"],"prefix":"10.1111","volume":"178","author":[{"given":"Huixia","family":"Bai","sequence":"first","affiliation":[{"name":"College of Life Sciences, Agriculture and Forestry Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafang","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Agriculture and Forestry Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Huan","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0817-4327","authenticated-orcid":false,"given":"Linlin","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Agriculture and Forestry Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Agriculture and Forestry Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Kong","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Agriculture and Forestry Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7526-7680","authenticated-orcid":false,"given":"Yushu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Agriculture and Forestry Qiqihar University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","DOI":"10.3390\/nu11020429","article-title":"Sorting Out the Value of Cruciferous Sprouts as Sources of Bioactive Compounds for Nutrition and Health","volume":"11","author":"Abell\u00e1n \u00c1.","year":"2019","journal-title":"Nutrients"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","DOI":"10.3390\/nu13082882","article-title":"Edible Plant Sprouts: Health Benefits, Trends, and Opportunities for Novel Exploration","volume":"13","author":"Aloo S. O.","year":"2021","journal-title":"Nutrients"},{"key":"e_1_2_11_4_1","first-page":"1","article-title":"Promising Kale (\n                     Brassica oleracea var. acephala\n                  ) Populations From Black Sea Region, Turkey","volume":"33","author":"Balkaya A.","year":"2005","journal-title":"New Zealand Journal of Experimental Agriculture"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1021\/jf047945a","article-title":"Direct Antioxidant Activity of Purified Glucoerucin, the Dietary Secondary Metabolite Contained in Rocket (\n                     Eruca sativa\n                   Mill.) Seeds and Sprouts","volume":"53","author":"Barillari J.","year":"2005","journal-title":"Journal of Agricultural & Food Chemistry"},{"key":"e_1_2_11_6_1","first-page":"2310","article-title":"Glucosinolate Profiling and Antimicrobial Screening of Aurinia leucadea (Brassicaceae)","volume":"8","author":"Bla\u017eevi\u0107 I.","year":"2011","journal-title":"Food Chemistry"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.foodchem.2011.11.086","article-title":"Evaluation of the Antioxidant Potential of Cauliflower (\n                     Brassica oleracea\n                  ) From a Glucosinolate Content Perspective","volume":"132","author":"Cabello\u2010Hurtado F.","year":"2012","journal-title":"Food Chemistry"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1177\/1082013211433077","article-title":"Bioactive Compounds and Phenolic\u2010Linked Functionality of Powdered Tropical Fruit Residues","volume":"18","author":"Correia R. T.","year":"2012","journal-title":"Food Science and Technology International"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","DOI":"10.3390\/agronomy10091424","article-title":"Sprouts and Microgreens: Trends, Opportunities, and Horizons for Novel Research","volume":"10","author":"Galieni A.","year":"2020","journal-title":"Agronomy"},{"key":"e_1_2_11_10_1","doi-asserted-by":"crossref","DOI":"10.3390\/molecules24193537","article-title":"Effect of Methyl Jasmonate on Phenolic Accumulation in Wounded Broccoli","volume":"24","author":"Guan Y.","year":"2019","journal-title":"Molecules"},{"key":"e_1_2_11_11_1","first-page":"41176","article-title":"Jasmonate\u2010Regulated Root Growth Inhibition and Root Hair Elongation","volume":"74","author":"Han X.","year":"2023","journal-title":"Journal of Experimental Botany"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/j.devcel.2010.10.024","article-title":"DELLAs Modulate Jasmonate Signaling via Competitive Binding to JAZs","volume":"19","author":"Hou X. L.","year":"2010","journal-title":"Developmental Cell"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","DOI":"10.3390\/foods10051032","article-title":"Ontogenetic Variation in the Mineral, Phytochemical and Yield Attributes of Brassicaceous Microgreens","volume":"10","author":"Kyriacou M. C.","year":"2021","journal-title":"Food"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","DOI":"10.1093\/plphys\/kiaf546","article-title":"The Reason for Kale Reddening in Late Autumn Is BoCBF2\/BoMYB2 Synergistically Regulate BoDFR Expression","volume":"199","author":"Li X.","year":"2025","journal-title":"Plant Physiology"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real\u2010Time Quantitative PCR and the 2(\u2010Delta Delta C(T)) Method","volume":"25","author":"Livak K. J.","year":"2001","journal-title":"Methods"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","DOI":"10.3390\/molecules28041840","article-title":"Antioxidant and Antiproliferative Activities of Kale (\n                     Brassica oleracea\n                   L. var. acephala DC.) and Wild Cabbage (\n                     Brassica incana\n                   Ten.) Polyphenolic Extracts","volume":"28","author":"Lu\u010di\u0107 D.","year":"2023","journal-title":"Molecules"},{"key":"e_1_2_11_17_1","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1007\/s12298-024-01513-x","article-title":"Effect of Sodium Selenite on the Synthesis of Glucosinolates and Antioxidant Capacity in Chinese Cabbage (\n                     Brassica rapa\n                   L.ssp.pekinensis)","volume":"30","author":"Luo Y. F.","year":"2024","journal-title":"Physiology and Molecular Biology of Plants"},{"key":"e_1_2_11_18_1","doi-asserted-by":"crossref","DOI":"10.3390\/nu12030868","article-title":"Brassicaceae\u2010Derived Anticancer Agents: Towards a Green Approach to Beat Cancer","volume":"12","author":"Mandrich L.","year":"2020","journal-title":"Nutrients"},{"key":"e_1_2_11_19_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.envexpbot.2009.01.002","article-title":"Methyl Jasmonate\u2010Induced Antioxidant Defence in Root Apoplast From Sunflower Seedlings","volume":"66","author":"Maria C. P. 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A.","year":"2008","journal-title":"Journal of the Science of Food and Agriculture"},{"key":"e_1_2_11_23_1","doi-asserted-by":"crossref","first-page":"50717","DOI":"10.1074\/jbc.M407681200","article-title":"Desulfoglucosinolate Sulfotransferases From \n                     Arabidopsis thaliana\n                   Catalyze the Final Step in the Biosynthesis of the Glucosinolate Core Structure","volume":"279","author":"Piotrowski M.","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_11_24_1","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1080\/10408398.2018.1454400","article-title":"Kale (\n                     Brassica oleracea var. acephala\n                  ) as a Superfood: Review of the Scientific Evidence Behind the Statement","volume":"59","author":"\u0160amec D.","year":"2018","journal-title":"Critical Reviews in Food Science and Nutrition"},{"key":"e_1_2_11_25_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00425-017-2705-9","article-title":"Biosynthesis and Bioactivity of Glucosinolates and Their Production in Plant In\u00a0Vitro Cultures","volume":"246","author":"S\u00e1nchez\u2010Pujante P. 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S.","year":"2012","journal-title":"Trends in Plant Science"},{"key":"e_1_2_11_29_1","first-page":"1021","article-title":"Jasmonates: Biosynthesis, Perception, Signal Transduction and Action in Plant Stress Response, Growth and Development","volume":"111","author":"Wasternack C.","year":"2013","journal-title":"Annals of Botany"},{"key":"e_1_2_11_30_1","first-page":"1303","article-title":"Jasmonates: Biosynthesis, Metabolism, and Signaling by Proteins Activating and Repressing Transcription","volume":"68","author":"Wasternack C.","year":"2017","journal-title":"Journal of Experimental Botany"},{"key":"e_1_2_11_31_1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2019.01349","article-title":"The Crosstalks Between Jasmonic Acid and Other Plant Hormone Signaling Highlight the Involvement of Jasmonic Aid as a Core Component in Plant Response to Biotic and Abiotic Stresses","volume":"10","author":"Yang J.","year":"2019","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_11_32_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.biotechadv.2005.01.003","article-title":"Elicitor Signal Transduction Leading to Production of Plant Secondary Metabolites","volume":"23","author":"Zhao J.","year":"2005","journal-title":"Biotechnology Advances"},{"key":"e_1_2_11_33_1","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1146\/annurev-arplant-042916-040856","article-title":"Trade\u2010Offs Between Plant Growth and Defense Against Insect Herbivory: An Emerging Mechanistic Synthesis","volume":"68","author":"Z\u00fcst T.","year":"2017","journal-title":"Annual Review of Plant Biology"}],"container-title":["Physiologia Plantarum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.71094","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ppl.71094","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ppl.71094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:33:27Z","timestamp":1788345207000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppl.71094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/ppl.71094"],"URL":"https:\/\/doi.org\/10.1111\/ppl.71094","archive":["Portico"],"relation":{},"ISSN":["0031-9317","1399-3054"],"issn-type":[{"value":"0031-9317","type":"print"},{"value":"1399-3054","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71094"}}