{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T15:36:18Z","timestamp":1793201778067,"version":"4.5.0"},"reference-count":77,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["402255\/2023\u20102"],"award-info":[{"award-number":["402255\/2023\u20102"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Recurrent drought limits the productivity of perennial crops such as citrus, whose tolerance to water deficit largely depends on scion\u2013rootstock interactions. Although physiological responses to recurrent drought are well documented, the epigenetic states associated with these responses remain poorly understood, limiting the use of epigenetic variation to improve drought resilience. Here, we investigated whole\u2010genome DNA methylation profiles and transcriptional responses in \u201cPera\u2010D6\u201d sweet orange (LP) grafted onto two rootstocks with contrasting physiological strategies for coping with water deficit: \u201cRangpur\u201d lime (RL), associated with dehydration avoidance through enhanced water acquisition, and \u201cSunki Maravilha\u201d mandarin (SM), associated with tighter regulation of transpiration and physiological adjustments under stress. Grafted plants were exposed to three cycles of water deficit followed by rehydration. In the scion, we detected rootstock\u2010dependent methylation dynamics, with LP\/RL exhibiting broader patterns of methylation change and LP\/SM showing a more targeted response. In the LP\/SM combination, drought\u2010associated differentially methylated regions were linked to genes involved in photosynthesis, stomatal regulation, and transcriptional processes. For several stress\u2010responsive genes, promoter methylation changes coincided with qualitative trends in gene expression; however, no significant methylation\u2013expression correlations were detected after multiple\u2010testing correction. These findings indicate that recurrent drought is associated with rootstock\u2010dependent persistent DNA methylation patterns with potential functional relevance. Overall, the results highlight the importance of considering epigenetic variation in studies of drought response and suggest that rootstock selection may play a role in shaping regulatory responses to water deficit in citrus.<\/jats:p>","DOI":"10.1111\/ppl.71113","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:30:41Z","timestamp":1790119841000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Persistent Epigenetic Reprogramming Is Associated With Contrasting Drought\u2010Response Strategies in Citrus Scion\u2013Rootstock Systems"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1967-7703","authenticated-orcid":false,"given":"Monique Ayala Ara\u00fajo","family":"da Silva","sequence":"first","affiliation":[{"name":"Departamento de Biologia, Centro de Gen\u00e9tica e Biologia Molecular Universidade Estadual de Santa Cruz  Ilh\u00e9us Bahia 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Institut Agro  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4667-5535","authenticated-orcid":false,"given":"Maur\u00edcio Ant\u00f4nio","family":"Coelho\u2010Filho","sequence":"additional","affiliation":[{"name":"Embrapa Mandioca e Fruticultura  Cruz das Almas Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7590-0455","authenticated-orcid":false,"given":"Abelmon da Silva","family":"Gesteira","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Centro de Gen\u00e9tica e Biologia Molecular Universidade Estadual de Santa Cruz  Ilh\u00e9us Bahia Brazil"},{"name":"Embrapa Mandioca e Fruticultura  Cruz das Almas Bahia 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