{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:49:44Z","timestamp":1792039784248,"version":"4.4.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202445"],"award-info":[{"award-number":["32202445"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sodium hydrosulfide (NaHS), a hydrogen sulfide donor, has been reported to regulate postharvest senescence and quality maintenance in horticultural crops. However, its role in sand pear softening and the underlying physiological basis remain unclear. Mature fruits of the climacteric sand pear cultivar \u201cSucui No. 1\u201d were treated with NaHS and stored at 20\u00b0C to evaluate its effects on postharvest quality, ethylene production, cell wall metabolism, and softening\u2010related gene expression. Treatment with 1\u2009mmol\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    NaHS delayed firmness loss and reduced ethylene production, with limited effects on fruit color, weight loss, and total soluble solids during storage. NaHS\u2010treated fruit retained significantly higher firmness than control fruit from Day 15 onward, with the largest difference between treatments being 3.25\u2009N on Day 15. NaHS\u2010treated fruit retained higher levels of cellulose, hemicellulose, covalently soluble pectin, and ionically soluble pectin, and accumulated less water\u2010soluble pectin, indicating delayed cell wall polysaccharide disassembly. Consistently, NaHS suppressed the activities of several cell wall\u2010modifying enzymes, including cellulase, \u03b2\u2010glucosidase, xyloglucan endotransglucosylase\/hydrolase, polygalacturonase, and pectin methylesterase. RNA sequencing and qRT\u2010PCR analyzes showed that NaHS modulated the expression of genes associated with cell wall remodeling, ethylene biosynthesis, sulfur\u2010related metabolism, and transcriptional regulation, particularly during the early\u2010to\u2010middle storage stage. Transient silencing assays further indicated that the\n                    <jats:italic>XTH<\/jats:italic>\n                    gene (\n                    <jats:italic>gene11051<\/jats:italic>\n                    ) was associated with cellulose\u2010hemicellulose remodeling, whereas the two\n                    <jats:italic>PME<\/jats:italic>\n                    genes (\n                    <jats:italic>gene26382<\/jats:italic>\n                    and\n                    <jats:italic>gene8920<\/jats:italic>\n                    ) were associated with pectin solubilization. These results suggest that the delay in postharvest softening following NaHS treatment was associated with coordinated changes in ethylene production and cell wall disassembly.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71152","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:40:16Z","timestamp":1792039216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sodium Hydrosulfide Delays Postharvest Softening of Sand Pear Fruit in Association With Altered Ethylene Production and Cell Wall Metabolism"],"prefix":"10.1111","volume":"178","author":[{"given":"Jie","family":"Gao","sequence":"first","affiliation":[{"name":"College of Horticulture and Landscape Architecture, International Research Laboratory of Agriculture and Agri\u2010Product Safety, Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Ying","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, International Research Laboratory of Agriculture and Agri\u2010Product Safety, Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng\u2010Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, International Research Laboratory of Agriculture and Agri\u2010Product Safety, Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4642-7264","authenticated-orcid":false,"given":"Chun\u2010Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, International Research Laboratory of Agriculture and Agri\u2010Product Safety, Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7516-8527","authenticated-orcid":false,"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, International Research Laboratory of Agriculture and Agri\u2010Product Safety, Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2023.110114","article-title":"Recent Trends in Hydrogen\u2010Associated Treatments for Maintaining the Postharvest Quality of Fresh and Fresh\u2010Cut Fruits and Vegetables: A Review","volume":"156","author":"Ali M.","year":"2024","journal-title":"Food Control"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf034028g"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925\u20105214(02)00246\u20106"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1071\/FP05234","article-title":"Cell Wall Disassembly in Ripening Fruit","volume":"33","author":"Brummell D. 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