{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:41:46Z","timestamp":1792370506326,"version":"4.4.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004242","name":"Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","award":["PNURSP2026R214"],"award-info":[{"award-number":["PNURSP2026R214"]}],"id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004242","name":"Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n Methyl jasmonate (MeJA) is a commonly used elicitor for enhancing secondary metabolite production, including the anticancer terpenoid indole alkaloids (TIAs) vinblastine and vincristine. To investigate the physiological and molecular responses associated with MeJA\u2010induced TIA accumulation, we examined the effect of different MeJA concentrations on vinblastine and vincristine production, gene expression and antioxidant defence in\n \n Catharanthus roseus<\/jats:italic>\n <\/jats:styled-content>\n calli. MeJA significantly enhanced relative growth and increased vinblastine and vincristine accumulation, with the highest increases observed at 50\u2009\u03bcM MeJA, reaching 159.2% and 183.4% of control, respectively, and upregulated\n CrMPK3<\/jats:italic>\n ,\n STR<\/jats:italic>\n , and\n DAT<\/jats:italic>\n expression. The lower concentrations of MeJA showed higher H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n accumulation, DPPH free radical\u2010scavenging and flavonoid content. Whereas increased lipid peroxidation and PAL enzyme activity were associated with the higher concentrations (100 and 200\u2009\u03bcM). The upregulated expression of\n CrMPK3,<\/jats:italic>\n together with the biosynthetic genes\n STR<\/jats:italic>\n and\n DAT,<\/jats:italic>\n supports the involvement of MAPK in signalling responses that mediate MeJA\u2010elicited TIAs. Pearson correlation and principal component analysis showed that H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n accumulation was more closely associated with vinblastine and vincristine accumulation than with the expression of the investigated genes at the experimental time points. These findings suggest that H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n accumulation may be associated with downstream metabolic\/redox processes accompanying alkaloid accumulation, although an earlier transient role of H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n in MeJA signalling cannot be excluded. The enhanced flavonoid accumulation and DPPH scavenging activity further suggest a role for antioxidant responses in maintaining redox homeostasis during MeJA\u2010induced alkaloid accumulation.\n <\/jats:p>","DOI":"10.1111\/ppl.71157","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:17:36Z","timestamp":1792369056000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hydrogen Peroxide and\n MAPK<\/scp>\n \u2010Associated Responses During Methyl Jasmonate\u2010Induced Elicitation of Terpenoid Indole Alkaloid in\n Catharanthus roseus<\/i>\n Callus"],"prefix":"10.1111","volume":"178","author":[{"given":"Marwa Mostafa","family":"Rizk","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badreldin A.","family":"Hamed","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emad A.","family":"Alsherif","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9355-3952","authenticated-orcid":false,"given":"Shereen Magdy","family":"Korany","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science Princess Nourah bint Abdulrahman University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6426-2796","authenticated-orcid":false,"given":"Nadia Mohamed","family":"El\u2010Shafey","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2021.114298","article-title":"Role of Plant Biotechnology in Enhancement of Alkaloid Production From Cell Culture System of \n Catharanthus roseus\n : A Medicinal Plant With Potent Anti\u2010Tumor Properties","volume":"176","author":"Acharjee S.","year":"2022","journal-title":"Industrial Crops and Products"},{"key":"e_1_2_12_3_1","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2022.114634","article-title":"Expression of CrMPK3 and Alkaloid Synthesis Genes With Antioxidants in Callus of \n Catharanthus roseus\n in Response to Polyethylene Glycol","volume":"178","author":"Azadvari E.","year":"2022","journal-title":"Industrial Crops and Products"},{"issue":"1","key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","article-title":"Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels","volume":"44","author":"Beauchamp C.","year":"1971","journal-title":"Analytical Biochemistry"},{"issue":"11","key":"e_1_2_12_6_1","doi-asserted-by":"crossref","DOI":"10.3390\/molecules26113374","article-title":"Diversity in Chemical Structures and Biological Properties of Plant Alkaloids","volume":"26","author":"Bhambhani S.","year":"2021","journal-title":"Molecules"},{"key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/978-981-99-5736-1_23","volume-title":"Plant Physiology, Development and Metabolism","author":"Bhatla S. 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