{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:43:12Z","timestamp":1792672992017,"version":"4.5.0"},"reference-count":110,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":51,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiologia Plantarum"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Hypnum leptothallum<\/jats:italic>\n                    <\/jats:styled-content>\n                    (M\u00fcll. Hal.) Paris, a pioneer moss species thriving in karst rocky desertification areas, exhibits remarkable desiccation tolerance and holds promise for ecological restoration initiatives. Despite its potential, the species faces limitations due to its slow growth rate. This study contributes significantly to the understanding of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>H. leptothallum<\/jats:italic>\n                    <\/jats:styled-content>\n                    by systematically documenting the developmental process from spore germination to young gametophore formation and establishing an efficient in\u00a0vitro propagation system for widespread cultivation. Sequential developmental stages were identified through detailed morphological analysis, encompassing spore germination, protonemal differentiation, and gametophore formation. The culture system was optimized through singlefactor experiments and response surface methodology (RSM). Initial screening indicated that 0.2\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    kinetin (KT) was the most effective plant growth regulator. Further RSM optimization determined that a medium composed of \u22121\/2.67 Benecke + 0.2\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    KT at pH\u20095.6 produced optimal growth. This medium yielded a protonemal patch diameter of 1.12\u2009\u00b1\u20090.047\u2009mm, gametophore height of 1.97\u2009\u00b1\u20090.107\u2009mm, gametophore yield of 9.75\u2009\u00b1\u20090.855 numbers, coverage of 19.32%\u2009\u00b1\u20090.295%, and biomass of 0.17\u2009\u00b1\u20090.003\u2009g. By following this established protocol, a sufficient amount of protonema material is ensured to facilitate the rapid cultivation of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>H. leptothallum<\/jats:italic>\n                    <\/jats:styled-content>\n                    using fragmented protonema and additional nutrients. This method provides a practical means for the mass production of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>H. leptothallum<\/jats:italic>\n                    <\/jats:styled-content>\n                    crusts, potentially contributing significantly to the mitigation of rocky desertification in karst regions.\n                  <\/jats:p>","DOI":"10.1111\/ppl.71161","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:56:23Z","timestamp":1792670183000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Propagation and Gametophore Development of the Desiccation\u2010Tolerant Moss\n                    <i>Hypnum leptothallum<\/i>"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0876-0918","authenticated-orcid":false,"given":"Yihong","family":"Xiang","sequence":"first","affiliation":[{"name":"College of Life Sciences, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9822-9966","authenticated-orcid":false,"given":"Shasha","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4537-6449","authenticated-orcid":false,"given":"Xiaofan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7572-5458","authenticated-orcid":false,"given":"Shangmei","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6401-4052","authenticated-orcid":false,"given":"Jiaojiao","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Tea Sciences, Guizhou Key Laboratory of Agricultural Microbiology Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3639-1421","authenticated-orcid":false,"given":"Wei","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro\u2010Bioengineering Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"108","article-title":"Factors Affecting on In\u00a0Vitro Gametophyte Formation From Spore Culture of Four Moss Species","volume":"28","author":"Ahmed M. 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Under Cold Stress During the Early Vegetative Stage","volume":"36","author":"Jia Y.","year":"2017","journal-title":"Journal of Plant Growth Regulation"},{"key":"e_1_2_11_45_1","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1093\/pcp\/pcaa098","article-title":"An Evolutionarily Primitive and Distinct Auxin Metabolism in the Lycophyte \n                     Selaginella moellendorffii","volume":"61","author":"Kaneko S.","year":"2020","journal-title":"Plant and Cell Physiology"},{"key":"e_1_2_11_46_1","first-page":"637","article-title":"Simulated Study on the Effects of Smoke on \n                     Sphagnum\n                   Spore Germination","volume":"30","author":"Kang Y.","year":"2019","journal-title":"Chinese Journal of Applied Ecology"},{"key":"e_1_2_11_47_1","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s11240-017-1180-0","article-title":"Modeling Some Mineral Nutrient Requirements for Micropropagated Wild Apricot Shoot Cultures","volume":"129","author":"Kovalchuk I. 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