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Artificial insemination (<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>) has been reported in several wild feline species, but pregnancy rates are low, partially owing to failures of current hormonal stimulation protocols. Therefore, this study describes the application of reliable methods to monitor ovarian activity and the development of an effective hormonal protocol to induce oestrus and ovulation in <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>frican lions. Application of porcine <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> and porcine <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> was shown to be effective for inducing follicular growth and ovulation, and this regimen appeared to be superior to protocols described earlier in terms of ovulation and fertilization rates. Furthermore, non\u2010surgical <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content> was performed successfully in lions, and uterine\u2010stage embryos were collected and cryopreserved. <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>frican lions may serve as a valuable model to develop assisted reproduction for propagation of relic zoo populations in the critically endangered <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>sian lion or <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>arbary lion.<\/jats:p>","DOI":"10.1111\/rda.12026","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"156-160","source":"Crossref","is-referenced-by-count":16,"title":["Embryo Retrieval after Hormonal Treatment to Control Ovarian Function and Non\u2010surgical Artificial Insemination in <scp>A<\/scp>frican Lions (<i><scp>P<\/scp>anthera leo<\/i>)"],"prefix":"10.1111","volume":"47","author":[{"given":"F","family":"Goeritz","sequence":"first","affiliation":[{"name":"Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Painer","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Jewgenow","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Hermes","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Givskud Zoo Givskud Give 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