{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:40:28Z","timestamp":1761648028795},"reference-count":21,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Progesterone exerts its effect by binding to specific progesterone receptors (<jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>) within the cell. In dogs and cats, no data are available on <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> isoforms as found in other species. We therefore investigated the sequence of the <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> gene and encoded protein in dogs and cats, the expression of <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> isoforms in mammary tissue using <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blots and the presence of <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> in mammary tissue using immunohistochemistry. Comparison of the amino acid sequence of the canine and feline <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> with human <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> revealed major differences in the <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010specific upstream segment (<jats:styled-content style=\"fixed-case\">BUS<\/jats:styled-content>). However, the essential activation function 3 (<jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>3) domain was intact in the cat but mutated in the dog. The <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and ligand\u2010binding domains were highly similar among the species. In cats with fibroadenomatous hyperplasia (<jats:styled-content style=\"fixed-case\">FAH<\/jats:styled-content>), high expression of <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> together with growth hormone (<jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content> receptor (<jats:styled-content style=\"fixed-case\">GHR<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">IGF\u2010I<\/jats:styled-content> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was found in comparison with feline mammary carcinomas. Immunohistochemical analysis showed strong nuclear as well as cytoplasmic staining for <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">FAH<\/jats:styled-content>. Western blot analysis revealed expression of the <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> isoforms in the feline mammary gland. In canine mammary tissue, the most abundant <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> staining was found in proliferative zones of the mammary gland. Western blot analyses showed mainly staining for <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> with lower <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> staining. It is concluded that in dogs and cats both <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> isoforms are expressed. The role of mutations found in the canine <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> is discussed.<\/jats:p>","DOI":"10.1111\/rda.12045","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"313-317","source":"Crossref","is-referenced-by-count":11,"title":["Progesterone Receptor Isoforms in the Mammary Gland of Cats and Dogs"],"prefix":"10.1111","volume":"47","author":[{"given":"A","family":"Gracanin","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"de Gier","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Zegers","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Bominaar","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GR","family":"Rutteman","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AC","family":"Schaefers\u2010Okkens","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HS","family":"Kooistra","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JA","family":"Mol","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1721"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.09.021"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.6.1994-2008.2003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.03.004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2006.06.005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-007-0019-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1586\/eem.11.25"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb02412.x"},{"key":"e_1_2_7_10_1","unstructured":"GracaninA van WolferenME SartoriusCA BrenkmanAB SchoonenWG MolJA 2012:Loss of AF3 Domain Function in Canid Progesterone Receptor. Endocrinology accepted."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(00)00173-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2004.09.057"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.158.24.821"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117888"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1732707100"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"issue":"1","key":"e_1_2_7_17_1","first-page":"73","article-title":"Gene expression profiles of progestin\u2010induced canine mammary hyperplasia and spontaneous mammary tumors","volume":"60","author":"Rao NA","year":"2009","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1621\/nrs.07009"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.134.1.7506206"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-3251"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1027"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106843200"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12045","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:50:19Z","timestamp":1694649019000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":21,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12045"],"URL":"https:\/\/doi.org\/10.1111\/rda.12045","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}