{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:42:19Z","timestamp":1791924139767,"version":"4.3.4"},"reference-count":11,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of this study was to assess epididymal sperm characteristics and serum testosterone concentration in cats under natural photoperiod. The hypothesis was that natural photoperiod induces seasonal changes in spermatozoal quality and serum testosterone concentration. Mixed breed tomcats (n\u00a0=\u00a043) that underwent bilateral orchiectomy at a municipal public pet shelter were used in the study. Epididymides were divided into two groups according to time of castration. In Group I, toms were castrated during increasing light (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>; [winter and spring; n\u00a0=\u00a024]), and group <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>, during decreasing light (<jats:styled-content style=\"fixed-case\">DL<\/jats:styled-content>; [summer and fall; n\u00a0=\u00a019]). Only mature toms castrated in the two lasts weeks of each season were included in this study. Sperm samples were obtained by cutting the cauda epididymis in <jats:styled-content style=\"fixed-case\">Tris<\/jats:styled-content> solution and tested for motility (<jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>,% motile), velocity (<jats:styled-content style=\"fixed-case\">VEL<\/jats:styled-content>, 0\u20135), total sperm cells (<jats:styled-content style=\"fixed-case\">TS<\/jats:styled-content>, 10<jats:sup>6<\/jats:sup>), acrosome integrity (<jats:styled-content style=\"fixed-case\">ACR<\/jats:styled-content>,% intact; <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content>), plasma membrane integrity (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>,%intact; <jats:styled-content style=\"fixed-case\">CFDA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>) and sperm morphology (<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content>,% normal). Before orchiectomy, blood samples were taken to measure serum concentrations of testosterone (T2) by a solid\u2010phase <jats:styled-content style=\"fixed-case\">RIA<\/jats:styled-content>. Data were analysed with the mixed procedure of <jats:styled-content style=\"fixed-case\">SAS<\/jats:styled-content>. Toms castrated during <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> had higher sperm plasma membrane integrity and better sperm morphology compared to toms castrated during <jats:styled-content style=\"fixed-case\">DL<\/jats:styled-content> (69.0\u00a0\u00b1\u00a02.7 vs 60.6\u00a0\u00b1\u00a02.1, p\u00a0&lt;\u00a00.01; 45.9\u00a0\u00b1\u00a02.5 vs 35.9\u00a0\u00b1\u00a03.4; p\u00a0&lt;\u00a00.02; respectively) and tended to have higher sperm motility and total number of sperm cells compared to toms castrated during <jats:styled-content style=\"fixed-case\">DL<\/jats:styled-content> (56.3\u00a0\u00b1\u00a02.8 vs 47.3\u00a0\u00b1\u00a03.7, p\u00a0&lt;\u00a00.06; 13.8\u00a0\u00b1\u00a01.4 vs 10.0\u00a0\u00b1\u00a01.8, p\u00a0&lt;\u00a00.09). However, velocity, acrosome integrity and serum testosterone concentrations were similar between both groups (3.5\u00a0\u00b1\u00a00.1 vs 3.4\u00a0\u00b1\u00a00.1, p\u00a0&gt;\u00a00.6; 45.8\u00a0\u00b1\u00a03.3 vs 44.0\u00a0\u00b1\u00a04.0, p\u00a0&gt;\u00a00.72; 0.76\u00a0\u00b1\u00a00.15 vs 0.59\u00a0\u00b1\u00a00.19, p\u00a0&gt;\u00a00.51; respectively). In conclusion, natural photoperiod induces seasonal changes in sperm quality with a moderate variation in serum testosterone concentrations.<\/jats:p>","DOI":"10.1111\/rda.12050","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"232-234","source":"Crossref","is-referenced-by-count":19,"title":["Effect of Natural Photoperiod on Epididymal Sperm Quality and Testosterone Serum Concentration in Domestic Cat (<i><scp>F<\/scp>elis silvestris catus<\/i>)"],"prefix":"10.1111","volume":"47","author":[{"given":"R","family":"Nu\u00f1ez\u2010Favre","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Veterinarias Laboratorio de Reproducci\u00f3n Animal Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MC","family":"Bonaura","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Laboratorio de Reproducci\u00f3n Animal Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CM","family":"Tittarelli","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Laboratorio de Reproducci\u00f3n Animal Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Mansilla\u2010Hermann","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Laboratorio de Reproducci\u00f3n Animal Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RL","family":"de la Sota","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Laboratorio de Reproducci\u00f3n Animal Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MA","family":"Stornelli","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Laboratorio de Reproducci\u00f3n Animal Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00780.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00817.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01141.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.010652"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1984.tb07220.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(84)90021-6"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1139\/z85-192"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/074873001129002051"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.188"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01448.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01449.x"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12050","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:47:46Z","timestamp":1694648866000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":11,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12050"],"URL":"https:\/\/doi.org\/10.1111\/rda.12050","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}