{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:46:32Z","timestamp":1759941992454},"reference-count":13,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"Contents<\/jats:title>The luteal phase on pregnant and non\u2010pregnant bitches is characteristic of this species and resembles significantly with respect to the growth pattern and luteal regression. Histological and immunostaining studies of the corpus luteum (CL<\/jats:styled-content>) may help to elucidate differences between the CL<\/jats:styled-content> of pregnant and non\u2010pregnant bitches. The purpose of this study was to characterize histologically and localize by immunohistochemistry the cell proliferation (K<\/jats:styled-content>i\u201067) and vascular endothelial growth (VEGF<\/jats:styled-content>) factors in the CL<\/jats:styled-content> of pregnant and non\u2010pregnant bitches. Eighteen bitches were analysed and distributed into three groups: In group I<\/jats:styled-content> (gestational diestrous), seven bitches were subjected to two inseminations at 4 and 6\u00a0days after the pre\u2010ovulatory LH<\/jats:styled-content> surge and ovariohysterectomized (OSH<\/jats:styled-content>) at 8\u201321\u00a0days after the first insemination. In group II<\/jats:styled-content> (cyclic diestrous; control), 6 (K<\/jats:styled-content>i\u201067) or 8 (VEGF<\/jats:styled-content>) bitches that were determined as non\u2010pregnant were OSH<\/jats:styled-content> at 12\u201325\u00a0days of the pre\u2010ovulatory LH<\/jats:styled-content> surge. In group III<\/jats:styled-content> (late pregnancy), three bitches had their ovary removed during caesarean section at 62\u201364\u00a0days after the pre\u2010ovulatory LH<\/jats:styled-content> surge. Portions of the ovarian cortex containing CL<\/jats:styled-content>s were cut and stored for histological and immunohistochemical analysis. Histological evaluation of the ovarian cortex showed a marked similarity in the morphological pattern among the CL<\/jats:styled-content>s in all three groups. The morphology and expression pattern of VEGF<\/jats:styled-content> and K<\/jats:styled-content>i\u201067 factors in CL<\/jats:styled-content>s of cyclic and gestational diestrous bitches were similar but significantly lower than that of late pregnant bitches (p\u00a0<\u00a00.05).<\/jats:p>","DOI":"10.1111\/rda.12056","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"61-65","source":"Crossref","is-referenced-by-count":2,"title":["Histological Characterization and Immunohistochemical Localization of Ki\u201067 and VEGF Factors in Bitches' Corpus Luteum at Cyclic and Early and Late Gestational Diestrous"],"prefix":"10.1111","volume":"47","author":[{"given":"AA","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction and Veterinary Radiology College of Veterinary Medicine and Animal Science UNESP Botucatu Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction and Veterinary Radiology College of Veterinary Medicine and Animal Science UNESP Botucatu Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AAP","family":"Derussi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction and Veterinary Radiology College of Veterinary Medicine and Animal Science UNESP Botucatu Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RB","family":"Ferioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinics College of Veterinary Medicine and Animal Science UNESP Botucatu Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Laufer\u2010Amorim","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinics College of 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