{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:15:20Z","timestamp":1779884120882,"version":"3.53.1"},"reference-count":0,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Fas is a membrane\u2010bound protein which upon activation causes programmed cell death. Fas ligand (FasL) binds Fas on target cells. Both these factors are known to regulate apoptosis at implantation in different species and thus might be involved in the regulation of implantation in dogs. The aim of the study was to assess the expression of Fas and FasL in canine uterine tissue throughout pregnancy as well as in pre\u2010implantation embryos using RT\u2010PCR and RT\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Uterine tissues was collected from of 21 healthy pregnant bitches (group I: days 10\u201312, n\u00a0=\u00a05; group II: days 18\u201325, n\u00a0=\u00a06; group III: days 28\u201345, n\u00a0=\u00a06) and from 4 non\u2010pregnant bitches (controls: days 10\u201312). Pregnancy stage was determined by days after mating, that is, 2\u20133\u00a0days after ovulation as determined by vaginal cytology and progesterone measurement. After ovariohysterectomy, uteri from group I bitches were flushed with PBS and the embryos washed and stored frozen at \u221280\u00b0. Tissues from the other groups were taken from the implantation and placentation sites, respectively, covered with Tissue Tek<jats:sup>\u00ae<\/jats:sup> and frozen at \u221280\u00b0. Extraction of RNA was performed with Trizol Reagent and RT\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> using SYBR green probes. In pre\u2010implantation embryos, only FasL but not Fas could be detected. In all tissues from pregnant and non\u2010pregnant bitches, both parameters were detectable. Before implantation (group I) expression of FasL resembled that of non\u2010pregnant bitches in early dioestrus and decreased significantly during implantation and thereafter (p\u00a0&lt;\u00a00.05). Expression of Fas did not change significantly until day 45. The relative expression of Fas exceeded that of FasL at each stage investigated, which is comparable to observations of other species; however, high standard deviations indicate high individual differences. These preliminary results point towards a regulatory function of the Fas\/FasL system during early canine pregnancy.<\/jats:p>","DOI":"10.1111\/rda.12063","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"169-172","source":"Crossref","is-referenced-by-count":8,"title":["Is Apoptosis a Regulatory Mechanism During Early Canine Pregnancy?"],"prefix":"10.1111","volume":"47","author":[{"given":"S","family":"Sch\u00e4fer\u2010Somi","sequence":"first","affiliation":[{"name":"Centre for Artificial Insemination and Embryo Transfer University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Sabitzer","sequence":"additional","affiliation":[{"name":"Vetomics\u2013Core Facility for Research University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Klein","sequence":"additional","affiliation":[{"name":"Vetomics\u2013Core Facility for Research University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Tomaszewski","sequence":"additional","affiliation":[{"name":"Centre for Artificial Insemination and Embryo Transfer University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Kanca","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Ankara Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HB","family":"Beceriklisoy","sequence":"additional","affiliation":[{"name":"Clinic for Obstetrics and Reproductive Diseases Faculty of Veterinary Medicine University of Adnan Menderes Ayd\u0131n Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Kucukaslan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Dicle Diyarbak\u0131r Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Agaoglu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Mehmet Akif Ersoy Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Kafkas Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ay","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Ondouz Mayis Samsun Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Aslan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Ankara Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12063","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:47:24Z","timestamp":1694648844000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":0,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12063"],"URL":"https:\/\/doi.org\/10.1111\/rda.12063","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}