{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T07:23:02Z","timestamp":1695972182268},"reference-count":7,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of this study was to assess the histological and ultrastructural changes in cat epididymides (n\u00a0=\u00a022) stored at 4\u00b0C in two different media [saline solution (<jats:styled-content style=\"fixed-case\">SAL<\/jats:styled-content>) or tris\u2010egg yolk (<jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content>)]. Our hypothesis was that epididymides stored in <jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content> would have delayed epithelial cell autolysis. Four epididymides were fixed and processed immediately, and the remaining 18 epididymides were stored at 4\u00b0C in <jats:styled-content style=\"fixed-case\">SAL<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content> for 24, 48 or 72\u00a0h. In histological sections, the nuclear features and stereocilia morphology were scored from 0 to 3. Ultrastructurally, nuclear chromatin and stereocilia morphology were scored from 0 to 3. In addition, using transmission electron microscopy nuclear number, nuclear area, mitochondrial number and mitochondrial area were recorded. In the histological study, parameters changed with time and media (p\u00a0&lt;\u00a00.01). A significant effect of time was observed (p\u00a0&lt;\u00a00.01), and the morphological changes were greatest when the storage time increased. Morphological changes were higher in <jats:styled-content style=\"fixed-case\">SAL<\/jats:styled-content> compared with <jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content> (p\u00a0&lt;\u00a00.01). In the ultrastructural study, nuclear chromatin and stereocilia morphology decreased with time and media as in the histological study (p\u00a0&lt;\u00a00.01). In addition, nuclear number and nuclear area changed with time (p\u00a0&lt;\u00a00.004; p\u00a0&lt;\u00a00.001) but not with media. Conversely, mitochondrial number and mitochondrial area did not change with media or time (p\u00a0&gt;\u00a00.05). In conclusion, these results show that <jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content> preserved epididymal epithelial cells better than <jats:styled-content style=\"fixed-case\">SAL<\/jats:styled-content>; this finding could help improve sperm quality of stored epididymides.<\/jats:p>","DOI":"10.1111\/rda.12073","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"281-283","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Storage Media and Storage Time on Histological and Ultrastructural Changes in Cat Epididymal Cells"],"prefix":"10.1111","volume":"47","author":[{"given":"CM","family":"Tittarelli","sequence":"first","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SB","family":"Jurado","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Nu\u00f1ez\u2010Favre","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MC","family":"Bonaura","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RL","family":"de la Sota","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MA","family":"Stornelli","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"7","article-title":"Ultrastructural study on the epithelium lining ductus pididymis in adult cats (Felis catus)","volume":"97","author":"Arrighi S","year":"1986","journal-title":"Arch Biol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.45.151"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.07.023"},{"key":"e_1_2_8_5_1","first-page":"1","volume-title":"Pathological Basis of Veterinary Disease","author":"Myers RK","year":"2007"},{"key":"e_1_2_8_6_1","volume-title":"SAS\u00ae Users Guide: Version 6","author":"SAS","year":"1989"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19980201)280:2<189::AID-JEZ10>3.0.CO;2-H"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.021"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12073","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:47:57Z","timestamp":1694648877000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":7,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12073"],"URL":"https:\/\/doi.org\/10.1111\/rda.12073","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}