{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:14:30Z","timestamp":1792170870816,"version":"4.4.0"},"reference-count":14,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of this study was to evaluate powdered coconut water extender (<jats:styled-content style=\"fixed-case\">ACP<\/jats:styled-content>\u2010106c; ACP Servi\u00e7os Tecnol\u00f3gicos Ltda, ACP Biotecnologia, Fortaleza, Cear\u00e1, Brazil) as a diluent for freezing dog semen and the fertility after vaginal insemination of semen frozen therein. Ten ejaculates were collected from five dogs, evaluated fresh, diluted in <jats:styled-content style=\"fixed-case\">ACP<\/jats:styled-content>\u2010106c, 10% egg yolk and 6% glycerol, cooled and frozen. In the first phase of the study, straws with frozen semen were thawed and immediately subjected to the same analysis as the fresh semen and, in addition, to <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>omputer\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>ssisted <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>emen <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>nalysis (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>). In phase 2, 10 bitches that had been subjected to natural breeding during a preceding oestrous cycle were vaginally inseminated with thawed semen that had been re\u2010diluted in <jats:styled-content style=\"fixed-case\">ACP<\/jats:styled-content>\u2010106c. After thawing, a mean of 77% sperm motility was obtained through subjective analysis and 77.3% through <jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>. Following artificial insemination, a 60% pregnancy rate was observed, resulting in a 50% parturition rate and a mean litter size of 3.4 (SEM 0.6), with 47.1% males and 52.9% females. <jats:styled-content style=\"fixed-case\">ACP<\/jats:styled-content>\u2010106c can be successfully used for freezing canine semen, and vaginal deposition of such semen yields similar pregnancy rates to those reported in other studies.<\/jats:p>","DOI":"10.1111\/rda.12077","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"289-292","source":"Crossref","is-referenced-by-count":16,"title":["Intravaginal Artificial Insemination in Bitches Using Frozen\/Thawed Semen after Dilution in Powdered Coconut Water (<scp>ACP<\/scp>\u2010106c)"],"prefix":"10.1111","volume":"47","author":[{"given":"DC","family":"Uchoa","sequence":"first","affiliation":[{"name":"Grande Canaf\u00edstula Kennel\/Inseminar Reprodu\u00e7\u00e3o Animal Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TFP","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Carnivore Reproduction Veterinary School State University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AC","family":"Mota Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Carnivore Reproduction Veterinary School State University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LDM","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Carnivore Reproduction Veterinary School State University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1209","article-title":"Cryopreservation of canine semen using a powdered coconut water extender (ACP\u2010106\u00ae): effect of glycerol temperature addition (27 and 4\u00b0C)","volume":"10","author":"Barbosa CC","year":"2009","journal-title":"Ci\u00eancia Animal Brasileira"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(90)90586-I"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00703.x"},{"key":"e_1_2_8_5_1","first-page":"96","volume-title":"Small Animal Reproduction and Infertility","author":"Concannon PW","year":"1986"},{"key":"e_1_2_8_6_1","first-page":"325","article-title":"Canine artificial insemination with frozen semen: comparison of intravaginal and intrauterine deposition of semen","volume":"47","author":"Fontbonne A","year":"1993","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.05.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.021"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2009.06.007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00630.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.12.009"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.138.7.154"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.022"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.11.029"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12077","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:49:35Z","timestamp":1694648975000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":14,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12077"],"URL":"https:\/\/doi.org\/10.1111\/rda.12077","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}