{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:12:39Z","timestamp":1777605159116,"version":"3.51.4"},"reference-count":5,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"Contents<\/jats:title>The most widely used screening test for the diagnosis of brucellosis in the dog is the rapid slide agglutination test in the presence of 2\u2010mercaptoethanol (2ME<\/jats:styled-content>\u2010RSAT<\/jats:styled-content>). The diagnosis is partially confirmed by the agar gel immunodiffusion test (AGID<\/jats:styled-content>) and definitively confirmed by bacteriological isolation. Some chronic cases not detected by these tests may be detected by ELISA<\/jats:styled-content> tests. The use of 2ME<\/jats:styled-content>\u2010RSAT<\/jats:styled-content> in routine clinical practice requires a microscope and an experienced operator. An immunochromatographic diagnostic test for canine brucellosis (FAST<\/jats:styled-content>est\u00ae<\/jats:bold><\/jats:sup> B<\/jats:styled-content>rucella c<\/jats:italic>., M<\/jats:styled-content>egacor, H<\/jats:styled-content>\u00f6rbranz, A<\/jats:styled-content>ustria) has been recently released. In this study, we compared the diagnostic performance of the FAST<\/jats:styled-content>est with those of 2ME<\/jats:styled-content>\u2010RSAT<\/jats:styled-content>, AGID<\/jats:styled-content> and ELISA<\/jats:styled-content>s. Sera from 17 healthy dogs used as negative controls yielded negative results by FAST<\/jats:styled-content>est, indicating a 100% specificity in this sample. Among 27 sera of dogs with acute or subacute brucellosis confirmed by B<\/jats:styled-content>.\u00a0canis<\/jats:italic> isolation, all of which were positive by RSAT<\/jats:styled-content> and ELISA<\/jats:styled-content>s, the FAST<\/jats:styled-content>est was positive in 24 cases and AGID<\/jats:styled-content> in 23. In acute and subacute cases, the sensitivity of FASTest was 89%. Sera from six dogs with bacteriologically confirmed chronic brucellosis, which were positive by ELISA<\/jats:styled-content>s but negative by 2ME<\/jats:styled-content>\u2010RSAT<\/jats:styled-content>, were also tested; 1 was positive by FAST<\/jats:styled-content>est and 4 were positive by AGID<\/jats:styled-content>. These preliminary results indicate a good specificity of the FAST<\/jats:styled-content>est (100% in this sample) but an unacceptable sensitivity as a screening test. In cases with chronic brucellosis, the sensitivity of the FAST<\/jats:styled-content>est was lower than that of ELISA<\/jats:styled-content>s but this assay could make a good intermediate test to be run after a positive RSAT<\/jats:styled-content> and before running an AGID<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/rda.12108","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"370-372","source":"Crossref","is-referenced-by-count":18,"title":["Preliminary Study of an Immunochromatography Test for Serological Diagnosis of Canine Brucellosis"],"prefix":"10.1111","volume":"47","author":[{"given":"MM","family":"Wanke","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Sciences Theriogenology Area University of Buenos Aires Buenos Aires Argentina"},{"name":"Faculty of Veterinary Sciences INITRA University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Cair\u00f3","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences Infectious Diseases Area University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Rossano","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences Infectious Diseases Area University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Lai\u00f1o","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences Infectious Diseases Area University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PC","family":"Baldi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy and Biochemistry IDEHU University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NE","family":"Monachesi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences Theriogenology Area University of Buenos Aires Buenos Aires Argentina"},{"name":"Faculty of Veterinary Sciences INITRA University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EA","family":"Comercio","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences Theriogenology Area University of Buenos Aires Buenos Aires Argentina"},{"name":"Faculty of Veterinary Sciences INITRA University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MM","family":"Vivot","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences Infectious Diseases Area University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"3","article-title":"A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M\u2010) organism as antigen","volume":"77","author":"Carmichael LE","year":"1987","journal-title":"Cornell Vet"},{"key":"e_1_2_8_3_1","first-page":"1","volume-title":"Ovine Epididymitis (Brucella ovis)","author":"OIE Manual of Standards for Diagnostic Tests and Vaccines","year":"2004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00152-9"},{"key":"e_1_2_8_5_1","unstructured":"WankeMM CairoF RossanoM Lai\u00f1oM BaldiPC Martinez VivotM 2011:Evaluation of a new commercial test for the rapid serological diagnosis of canine brucellosis. Brucellosis 2011 International Research Conference. Book of abstracts pp:120."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(82)90004-9"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:47:45Z","timestamp":1694648865000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":5,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12108"],"URL":"https:\/\/doi.org\/10.1111\/rda.12108","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}