{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T11:56:53Z","timestamp":1778587013968,"version":"3.51.4"},"reference-count":10,"publisher":"Wiley","issue":"s6","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Transcervical endometrial biopsy is a useful tool for obtaining information about uterine health in some species. The clinical application of information gained from histopathological interpretations of endometrial biopsies in the bitch has not been validated. We hypothesized that transcervical endometrial biopsy samples would be as diagnostic as full\u2010thickness uterine sections in identifying cystic endometrial hyperplasia (<jats:styled-content style=\"fixed-case\">CEH<\/jats:styled-content>), inflammation and periglandular fibrosis. Endometrial biopsies were obtained from 20 female adult dogs. Vaginal swabs, gross appearance of the vulva and vaginal tract, and serum progesterone values were used to determine the stage of the oestrous cycle at the time of sampling. The uteri were removed between 1 and 6\u00a0days after the biopsy procedure, and full\u2010thickness sections were collected from each uterine horn and ovary and processed for histopathology. Two pathologists, blinded to the origin of each sample, compared full\u2010thickness sections from the excised uteri to the biopsy samples collected via the transcervical technique. Pathologic features noted included: CEH, inflammation and periglandular fibrosis. Pathological diagnoses obtained from the biopsy sections were compared with those obtained from the full\u2010thickness sections, as well as comparing diagnoses between the two pathologists, using <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>c<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>emar's test. Of the 59 total biopsy samples obtained, 54 were considered diagnostic. All stages of the canine oestrous cycle were represented (anoestrus, proestrus, oestrus and dioestrus). Pyometra was not noted in any of the transcervical biopsy sections, but was noted in many of the full\u2010thickness sections collected from dogs in dioestrus, suggesting either that biopsy is not a sensitive indicator of pyometra or that the procedure may induce pyometra in dioestrous dogs. Transcervical endometrial biopsy showed similar sensitivity as full\u2010thickness sections in detecting <jats:styled-content style=\"fixed-case\">CEH<\/jats:styled-content>, inflammation and fibrosis. No differences in describing lesions were detected between pathologists.<\/jats:p>","DOI":"10.1111\/rda.12109","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T06:03:59Z","timestamp":1356329039000},"page":"342-346","source":"Crossref","is-referenced-by-count":31,"title":["Diagnostic Value of Transcervical Endometrial Biopsies in Domestic Dogs Compared with Full\u2010Thickness Uterine Sections"],"prefix":"10.1111","volume":"47","author":[{"given":"BW","family":"Christensen","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences Iowa State University Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DH","family":"Schlafer","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Cornell University Ithaca NY 14853 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DW","family":"Agnew","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health Michigan State University Lansing MI 48910 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Statistics Iowa State University Ames IA 50011 USA"},{"name":"Departments of Veterinary Diagnostic and Production Animal Medicine Iowa State University Ames IA 50011 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Kozlowski","sequence":"additional","affiliation":[{"name":"Research Department St. Louis Zoo St. Louis MO 63110 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CS","family":"Asa","sequence":"additional","affiliation":[{"name":"Research Department St. Louis Zoo St. Louis MO 63110 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2004.00489.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1090.2009.00107.x"},{"key":"e_1_2_7_4_1","first-page":"61","article-title":"Development of a technique for transcervical collection of uterine tissue in bitches","volume":"57","author":"G\u00fcnzel\u2010Apel AR","year":"2001","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.22233\/9781905319541.5","volume-title":"BSAVA Manual of Canine and Feline Reproduction and Neonatology","author":"Heimendahl A","year":"2010"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1638\/2010-0222.1"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.59.185"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.61.433"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms1939.52.979"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.041"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.036"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12109","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T23:48:47Z","timestamp":1694648927000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":10,"journal-issue":{"issue":"s6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/rda.12109"],"URL":"https:\/\/doi.org\/10.1111\/rda.12109","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}