{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T04:02:44Z","timestamp":1774584164647,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,10,26]],"date-time":"2012-10-26T00:00:00Z","timestamp":1351209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The expression of 12 different aquaporin subtypes in equine endometrium was examined at the m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>and protein level. Endometrial samples were obtained during anoestrus, oestrus, 8, and 14\u00a0days after ovulation in non\u2010pregnant mares, and 14\u00a0days after ovulation in pregnant mares. Quantitative<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>revealed a time\u2010dependent pattern for all aquaporin subtypes examined except for<jats:italic><jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>10<\/jats:italic>and<jats:italic>12<\/jats:italic>.<jats:italic><jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>3<\/jats:italic>,<jats:italic>5<\/jats:italic>and<jats:italic>7<\/jats:italic>showed highest m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>abundance 8\u00a0days after ovulation, while<jats:italic><jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>0<\/jats:italic>and<jats:italic>2<\/jats:italic>were most abundant at Day 14 of the cycle in non\u2010pregnant mares. At 14\u00a0days of pregnancy,<jats:italic><jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1<\/jats:italic>,<jats:italic>4<\/jats:italic>,<jats:italic>8<\/jats:italic>,<jats:italic>9<\/jats:italic>and<jats:italic>11<\/jats:italic>displayed highest expression levels. Western blot analysis confirmed protein expression of<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>0, 2 and 5. Immunohistochemistry localized protein expression to luminal and glandular epithelial and stromal cells.<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>0 staining intensity was highest in samples obtained on Day 14 of the oestrous cycle.<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>2 immunoreactivity seemed to be stronger in samples collected 14\u00a0days after ovulation from non\u2010pregnant animals, in particular luminal epithelial staining. Samples collected 8\u00a0days after ovulation from cyclic animals were characterized by intense<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>5 staining of glandular epithelium, predominantly in the deeper glands. Progesterone treatment of anoestrous mares did not enhance expression of<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s, indicating that factors other than progesterone are required for the up\u2010regulation of certain<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>subtypes during dioestrus. In conclusion, it seems that an equine\u2010specific collaboration of aquaporin subtypes contributes to changes in endometrial fluid content occurring throughout the oestrous cycle and contributes to endometrial receptivity during early pregnancy in the mare.<\/jats:p>","DOI":"10.1111\/rda.12116","type":"journal-article","created":{"date-parts":[[2012,10,26]],"date-time":"2012-10-26T11:32:51Z","timestamp":1351251171000},"page":"529-537","source":"Crossref","is-referenced-by-count":28,"title":["Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy"],"prefix":"10.1111","volume":"48","author":[{"given":"C","family":"Klein","sequence":"first","affiliation":[{"name":"Department of Veterinary Science MH Gluck Equine Research Center Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MHT","family":"Troedsson","sequence":"additional","affiliation":[{"name":"Department of Veterinary 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