{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:17Z","timestamp":1787016017511,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T00:00:00Z","timestamp":1355097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Contents<\/jats:title>\n \n The first objective of this study was to evaluate intrauterine nitric oxide (\n NO<\/jats:styled-content>\n ) and endometrial inducible NO synthase (i\n NOS<\/jats:styled-content>\n ) in mares susceptible or resistant to persistent breeding\u2010induced endometritis (\n PBIE<\/jats:styled-content>\n ) within 24\u00a0h after breeding. Mares susceptible (n\u00a0=\u00a06) or resistant (n\u00a0=\u00a06) to\n PBIE<\/jats:styled-content>\n were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24\u00a0h after insemination. Uterine secretions were analysed for\n NO<\/jats:styled-content>\n and biopsies were analyzed for i\n NOS<\/jats:styled-content>\n expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (\n MCWE<\/jats:styled-content>\n ) on uterine\n NO<\/jats:styled-content>\n production and endometrial i\n NOS<\/jats:styled-content>\n m\n RNA<\/jats:styled-content>\n expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50\u00a0mg of dexamethasone IV or (iii)\n MCWE IV<\/jats:styled-content>\n 24\u00a0h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for\n NO<\/jats:styled-content>\n and i\n NOS<\/jats:styled-content>\n m\n RNA<\/jats:styled-content>\n . In Experiment 1, resistant mares had an increase in i\n NOS<\/jats:styled-content>\n m\n RNA<\/jats:styled-content>\n expression 2\u00a0h post\u2010breeding compared to baseline (p\u00a0=\u00a00.045), 12\u00a0h (p\u00a0=\u00a00.014) and 24\u00a0h (p\u00a0=\u00a00.001). Susceptible mares had higher expression 2\u00a0h compared to 6\u00a0h (p\u00a0=\u00a00.046). No differences were observed in m\n RNA<\/jats:styled-content>\n or protein expression of i\n NOS<\/jats:styled-content>\n between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine\n NO<\/jats:styled-content>\n over 24\u00a0h, while susceptible mares had an increase over time, with a significantly higher increase in total\n NO<\/jats:styled-content>\n than resistant mares at 6 (p\u00a0=\u00a00.04) and 12\u00a0h (p\u00a0=\u00a00.032). In Experiment 2, no differences were observed for i\n NOS<\/jats:styled-content>\n m\n RNA<\/jats:styled-content>\n expression. Susceptible mares had increased\n NO<\/jats:styled-content>\n when compared to resistant mares (p\u00a0=\u00a00.008) and\n MCWE<\/jats:styled-content>\n decreased\n NO<\/jats:styled-content>\n (p\u00a0=\u00a00.047).\n <\/jats:p>","DOI":"10.1111\/rda.12124","type":"journal-article","created":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T06:37:15Z","timestamp":1355121435000},"page":"554-561","source":"Crossref","is-referenced-by-count":32,"title":["An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding\u2010Induced Endometritis and the Effects of Immunomodulation"],"prefix":"10.1111","volume":"48","author":[{"given":"EM","family":"Woodward","sequence":"first","affiliation":[{"name":"The Maxwell H. 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