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Therefore, a mechanism for separating virus from spermatozoa in the semen of carrier stallions would facilitate control measures for preventing disease transmission. In this study, the use of several modifications of single\u2010layer centrifugation (<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> SLC<\/jats:styled-content> with an inner tube and double <jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>) through <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>ndrocoll\u2010<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>, a species\u2010specific colloid were evaluated for their ability to separate spermatozoa from virus in ejaculates from carrier stallions. The three types of <jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content> significantly reduced the virus titre in fresh semen at 0\u00a0h and in stored semen at 24\u00a0h (p\u00a0&lt;\u00a00.001) but did not completely eliminate the virus. Sperm motility parameters such as total motility and progressive motility were significantly increased after colloid centrifugation, whereas curvilinear velocity and amplitude of lateral head deviation were decreased, and the remainder (straight line velocity, average path velocity, straightness, linearity, wobble and beat cross\u2010frequency) were not significantly affected by the processing. Although virus titres were reduced in the <jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content> samples, significant levels of infectivity still remained, especially in stallions shedding large amounts of virus. It remains to be determined whether <jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>\u2010processed sperm samples from stallions shedding low virus titres retain sufficient <jats:styled-content style=\"fixed-case\">e<\/jats:styled-content>quine arteritis virus to cause infection in mares through artificial insemination.<\/jats:p>","DOI":"10.1111\/rda.12133","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T12:38:28Z","timestamp":1354192708000},"page":"604-612","source":"Crossref","is-referenced-by-count":15,"title":["Single\u2010Layer Centrifugation Reduces Equine Arteritis Virus Titre in the Semen of Shedding Stallions"],"prefix":"10.1111","volume":"48","author":[{"given":"JM","family":"Morrell","sequence":"first","affiliation":[{"name":"Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Timoney","sequence":"additional","affiliation":[{"name":"Maxwell H. 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