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This would permit transport of samples collected remotely, to the laboratory (at 4\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>) for cryopreservation. Epididymal sperm from plains bison (n\u00a0=\u00a011) and ejaculated sperm from wood bison (n\u00a0=\u00a03) were collected, extended and held at 4\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>for extended periods of time. At intervals, an aliquot was cryopreserved. Post\u2010thaw sperm motion characteristics were evaluated by computer assisted sperm analysis. Representative plains bison sperm samples (n\u00a0=\u00a03) were evaluated for their<jats:italic>in vitro<\/jats:italic>fertilizing ability in a heterologous system using bovine oocytes. There was no statistical difference in total and progressive motility of plains bison epididymal sperm when cryopreserved after chilling for 24, 48 or 72\u00a0h. For wood bison ejaculated sperm, there was no difference in total and progressive motility for sperm cryopreserved following 24 or 48\u00a0h of chilling. However, one of the three bulls showed significantly poorer fertilization (based on cleavage rate) with sperm chilled for 72 compared to 24 and 48\u00a0h prior to freezing. In conclusion, plains bison epididymal sperm can be chilled for 72\u00a0h and wood bison ejaculated sperm can be chilled for at least 48\u00a0h prior to cryopreservation without compromising post\u2010thaw sperm motility, while heterologous<jats:italic>in vitro<\/jats:italic>fertilization (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>) assay indicated a between\u2010bull variation in the<jats:italic>in vitro<\/jats:italic>fertilizing ability of sperm chilled for an extended duration before cryopreservation.<\/jats:p>","DOI":"10.1111\/rda.12137","type":"journal-article","created":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T12:01:52Z","timestamp":1357128112000},"page":"636-642","source":"Crossref","is-referenced-by-count":8,"title":["Effect of Chilling Duration on Post\u2010Thaw Characteristics of Sperm from the<scp>N<\/scp>orth<scp>A<\/scp>merican bison (<i><scp>B<\/scp>ison 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