{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T03:16:24Z","timestamp":1770520584472,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T00:00:00Z","timestamp":1358294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Contents<\/jats:title>Total five ear skin fibroblast lines (named F<\/jats:styled-content>1, F<\/jats:styled-content>2, F3<\/jats:styled-content>, F<\/jats:styled-content>4 and F<\/jats:styled-content>5) from different newborn Holstein cows have been used as nuclear donor cells for producing cloned cows by somatic cell nuclear transfer (SCNT<\/jats:styled-content>). The effects of these cell lines on both in vitro<\/jats:italic> and in vivo<\/jats:italic> developmental rates of cloned embryos, post\u2010natal survivability and incidence of large offspring syndrome (LOS<\/jats:styled-content>) were examined in this study. We found that the different cell lines possessed the same capacity to support pre\u2010implantation development of cloned embryos, the cleavage and blastocyst formation rates ranged from 80.2\u00a0\u00b1\u00a00.9 to 84.5\u00a0\u00b1\u00a02.5% and 28.5\u00a0\u00b1\u00a00.9 to 33.3\u00a0\u00b1\u00a01.4%, respectively. However, their capacities to support the in vivo<\/jats:italic> development of SCNT<\/jats:styled-content> embryos showed significant differences (p\u00a0<\u00a00.05). The pregnancy rates at 90 and 240\u00a0day were significantly lower in groups F<\/jats:styled-content>2 (4.9% and 3.3%) and F<\/jats:styled-content>3 (5.4% and 5.4%) compared to groups F<\/jats:styled-content>1 (23.3% and 16.3%), F<\/jats:styled-content>4 (25.7% and 18.6%) and F<\/jats:styled-content>5 (25.9% and 19.8%) (p\u00a0<\u00a00.05). The cloning efficiency was significantly higher in group F<\/jats:styled-content>5 than those in group F<\/jats:styled-content>1, F<\/jats:styled-content>2, F<\/jats:styled-content>3 and F<\/jats:styled-content>4 (9.3% vs 4.1%, 1.2%, 2.0% and 5.0%, respectively, p\u00a0<\u00a00.05). Moreover, large offspring syndrome (LOS<\/jats:styled-content>) incidence in group F<\/jats:styled-content>5 was significantly lower than those in other groups (p\u00a0<\u00a00.05). All cloned offspring from cell line F<\/jats:styled-content>1, F<\/jats:styled-content>2, F<\/jats:styled-content>3 and F<\/jats:styled-content>4 showed LOS<\/jats:styled-content> and gestation length delay, while all cloned offspring from F5 showed normal birthweight and gestation length. We concluded that the nuclear donor cell lines have significant impact on the in vivo<\/jats:italic> development of cloned embryos and the incidence of LOS<\/jats:styled-content> in cloned calves.<\/jats:p>","DOI":"10.1111\/rda.12140","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T11:05:54Z","timestamp":1358334354000},"page":"660-664","source":"Crossref","is-referenced-by-count":23,"title":["Nuclear Donor Cell Lines Considerably Influence Cloning Efficiency and the Incidence of Large Offspring Syndrome in Bovine Somatic Cell Nuclear Transfer"],"prefix":"10.1111","volume":"48","author":[{"given":"J","family":"Liu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Yangling Shaanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Yangling Shaanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Su","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Yangling Shaanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Yangling Shaanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Quan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Yangling Shaanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Yangling Shaanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1057"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.6.1768"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00665-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20618"},{"key":"e_1_2_7_6_1","first-page":"261","article-title":"Ultrasonographic fetal well\u2010being assessment, neonatal and postpartum findings of cloned pregnancies in cattle: a preliminary study on 10 fetuses and calves","volume":"50","author":"Buczinski S","year":"2009","journal-title":"Can Vet 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