{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:52:49Z","timestamp":1767858769777,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T00:00:00Z","timestamp":1358294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>To evaluate and compare the efficacy of various extenders for the cryopreservation of epididymal cat spermatozoa, two experiments were planned. Bovine and equine commercial extenders in the experiment 1 and TRIS\u2013egg yolk\u2013based extenders in experiment 2 were separately studied since the number of sperm collected per cat is reduced. Epididymal sperm samples were packaged into 0.25\u2010ml straws and frozen. Vigour, motility, morphology, acrosome status, sperm viability and functional membrane integrity were assessed at collection, after cooling and after thawing, while DNA integrity was evaluated at 0\u2010 and 6\u2010h post\u2010thaw. Experiment 1 compared the effect of three non\u2010feline commercial extenders \u2013 based on TRIS\u2013egg yolk (Triladyl), egg\u2010yolk\u2010free medium (AndroMed) and skimmed milk\u2010egg yolk (Gent) \u2013 on the quality of frozen\u2010thawed epididymal cat sperm. Values for sperm motility and functional membrane integrity in cooled sperm diluted in Triladyl were higher (p\u00a0&lt;\u00a00.001) than those recorded for Andromed and Gent. Except sperm morphology, the other assessed characteristics showed significant higher values in frozen\u2010thawed sperm diluted in Triladyl than in Andromed and Gent extenders. Experiment 2 analysed the effects of three TRIS\u2013egg yolk\u2013based extenders, one non\u2010feline commercial (Triladyl) and the other two prepared using different monosaccharides (glucose and fructose), on freezing\u2010thawed sperm. Results showed that specifically prepared extenders for cryopreservation of feline spermatozoa performed better than the commercial extender Triladyl, although sperm quality during the freezing\u2010thawing process did not significantly differ associated with the type of monosaccharide (glucose vs fructose) added to the mentioned extenders. Although TRIS\u2013egg yolk\u2013based extenders prepared in experiment 2 improved sperm cryoprotection, Triladyl remains a good option for practitioners who, for ease of use and availability, prefer to work with commercial extenders.<\/jats:p>","DOI":"10.1111\/rda.12142","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T11:21:01Z","timestamp":1358335261000},"page":"665-672","source":"Crossref","is-referenced-by-count":20,"title":["Effect of Different Extenders on<i>In Vitro<\/i>Characteristics of Feline Epididymal Sperm During Cryopreservation"],"prefix":"10.1111","volume":"48","author":[{"given":"E","family":"Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CC","family":"P\u00e9rez\u2010Mar\u00edn","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Vizuete","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Comparative Pathology Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EI","family":"Ag\u00fcera","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Physiology and Immunology University of Cordoba Cordoba Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2003.11.003"},{"key":"e_1_2_7_3_1","first-page":"545","article-title":"Freezing of cat semen in straws with different glycerol levels containing tris extender","volume":"28","author":"Baran A","year":"2004","journal-title":"Turk J Vet Anim Sci"},{"key":"e_1_2_7_4_1","first-page":"311","article-title":"Intrauterine insemination with cat semen frozen with various extenders","volume":"35","author":"Baran A","year":"2011","journal-title":"Turk J Vet Anim Sci"},{"key":"e_1_2_7_5_1","first-page":"93","article-title":"Taurine and hypotaurine in spermatozoa and epididymal fluid of cats","volume":"57","author":"Buff S","year":"2001","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_7_6_1","first-page":"392","article-title":"Skim milk\u2010egg yolk based semen extender compensates for non\u2010enzymatic antioxidant activity loss during equine semen cryopreservation","volume":"6","author":"Bustamante Filho IC","year":"2009","journal-title":"Anim Reprod"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2010.09.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.12.009"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199409990256"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1993.tb01189.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.3.175"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.07.004"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(87)90112-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.01.008"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1002\/j.1939-4640.1990.tb03229.x","article-title":"Teratospermic and normospermic domestic cats: ejaculate traits, pituitary\u2010gonadal hormones, and improvement of spermatozoal motility and morphology after swim\u2010up processing","volume":"11","author":"Howard JG","year":"1990","journal-title":"J Androl"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.01.006"},{"key":"e_1_2_7_17_1","unstructured":"JohnstonSD OsborneCA LipowitzAJ 1988:Characterizations of seminal plasma prostatic fluid and bulbourethral gland secretions in the domestic cat. 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