{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:35:52Z","timestamp":1784133352926,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T00:00:00Z","timestamp":1369353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,12]]},"abstract":"Contents<\/jats:title>Effects of sperm\u2010TALP<\/jats:styled-content> (TALP<\/jats:styled-content>) on the quality of fresh\u2010extended and frozen\u2010thawed epididymal cat sperm were evaluated. The epididymides suspended in Tris\u2013glucose\u2013citrate solution (Tris), a conventional medium, and TALP<\/jats:styled-content> were cut into small pieces to recover epididymal sperm. In experiment 1, the sperm pellets remained after centrifugation were re\u2010suspended (1\u00a0:\u00a02, v\/v) in Tris and TALP<\/jats:styled-content>. The sperm quality in all four groups, that is, sperm retrieved with Tris (I and II<\/jats:styled-content>) or TALP<\/jats:styled-content> (III<\/jats:styled-content> and IV<\/jats:styled-content>) and diluted with Tris (I and III<\/jats:styled-content>) or TALP<\/jats:styled-content> (II<\/jats:styled-content> and IV<\/jats:styled-content>) was assessed. The sperm motility at the 0\u2010h incubation in TALP<\/jats:styled-content>\u2013TALP<\/jats:styled-content> was superior to that of the rest (p\u00a0<\u00a00.001 to p\u00a0=\u00a00.04). At the 2\u2010h incubation, the motility in Tris\/TALP<\/jats:styled-content>\u2013TALP<\/jats:styled-content> was greater than that in Tris\/TALP<\/jats:styled-content>\u2013Tris (p\u00a0\u2264\u00a00.001). In experiment 2, after centrifugation, the sperm pellets were added with freezing extenders and frozen. The thawed sperm previously retrieved from the epididymides with Tris and TALP<\/jats:styled-content> were allotted so as not to further diluted (Tris\/TALP<\/jats:styled-content>\u2013O) and to further diluted (1\u00a0:\u00a01, v\/v) with Tris (Tris\/TALP<\/jats:styled-content>\u2013Tris) and TALP<\/jats:styled-content> (Tris\/TALP<\/jats:styled-content>\u2013TALP<\/jats:styled-content>) and were evaluated the quality. At both incubation times, the motility of frozen\u2010thawed sperm recovered with TALP<\/jats:styled-content> (TALP<\/jats:styled-content>\u2013O\/Tris\/TALP<\/jats:styled-content>) was comparable with or significantly higher than that in the Tris groups (Tris\u2013O\/Tris\/TALP<\/jats:styled-content>; p\u00a0=\u00a00.003 to p\u00a0>\u00a00.05). The motility and viability of thawed sperm in Tris\u2013Tris were significantly decreased during the 2\u2010h incubation (p\u00a0=\u00a00.007 for the motility and p\u00a0=\u00a00.01 for the viability). In both experiments, neither type of diluent (Tris vs TALP<\/jats:styled-content>) nor incubation time (0 vs 2\u00a0h) significantly affected the sperm membrane integrity under hypo\u2010osmotic condition (p\u00a0>\u00a00.05). According to beneficial effects on the quality of fresh\u2010extended and frozen\u2010thawed sperm demonstrated, sperm\u2010TALP<\/jats:styled-content> could be used as an alternative medium for recovering sperm from the epididymides and for diluting epididymal sperm in the domestic cat.<\/jats:p>","DOI":"10.1111\/rda.12185","type":"journal-article","created":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T12:18:40Z","timestamp":1369397920000},"page":"912-917","source":"Crossref","is-referenced-by-count":8,"title":["Sperm\u2010TALP<\/scp>: An Alternative Extender for Retrieving and Diluting Epididymal Sperm in the Domestic Cat"],"prefix":"10.1111","volume":"48","author":[{"given":"K","family":"Buranaamnuay","sequence":"first","affiliation":[{"name":"Reproductive Biology Research Group Insititute of Molecular Biosciences Mahidol University Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080310307"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00144-4"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod44.5.806"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01218.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.12.009"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(99)00030-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00106-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12142"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01432.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930057"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00742-2"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.05.003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod38.5.1171"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00384-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00571-4"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.011106"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1071\/BI9691527","article-title":"Freezing of ram spermatozoa by the pellet method. 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