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Semen aliquots were diluted with Tris\u2013citric acid\u2013fructose\u2013egg yolk extender containing either 1.0\u00a0g\/l streptomycin and 0.6\u00a0g\/l benzylpenicillin (control) or a combination of gentamycin, tylosin, lincomycin and spectinomycin (<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20101: 0.25, 0.05, 0.15 and 0.3;<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20102: 0.5, 0.1, 0.3 and 0.6;<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20103: 1.0, 0.2, 0.6 and 1.2\u00a0g\/l). Samples were assessed for motility and membrane integrity by computer\u2010assisted sperm analysis immediately after dilution and at 24, 48 and 72\u00a0h of cooled storage. Morphologically, normal spermatozoa were determined, and bacterial culture was performed at 24 and 72\u00a0h.<jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ycoplasma spp<\/jats:italic>. were detected in 14 of 20 ejaculates (70%) with severe growth in 12 samples. A reduction but not total elimination of mycoplasma growth occurred in all<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>extenders with the most pronounced reduction in group<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20103 (control vs<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20101 and<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20102 p\u00a0&lt;\u00a00.05, control vs<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>\u20103 p\u00a0&lt;\u00a00.001). Ureaplasmas were detected in four ejaculates, and growth was reduced to the same extent in<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>and control extender. Progressive motility in all groups, total motility in groups<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>1\u20133 and percentage of membrane\u2010intact spermatozoa in groups<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>2 and 3 decreased slightly (p\u00a0&lt;\u00a00.05) over time. In conclusion, dilution of canine semen with<jats:styled-content style=\"fixed-case\">GTLS<\/jats:styled-content>extender has no major detrimental effects on spermatozoa during cooled storage. It reduced the growth but did not totally eliminate mycoplasmas and ureaplasmas from cooled\u2010stored dog semen.<\/jats:p>","DOI":"10.1111\/rda.12194","type":"journal-article","created":{"date-parts":[[2013,6,28]],"date-time":"2013-06-28T05:49:00Z","timestamp":1372398540000},"page":"961-966","source":"Crossref","is-referenced-by-count":8,"title":["Cooled Storage of Canine Semen:<i>in vitro<\/i>Effects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes"],"prefix":"10.1111","volume":"48","author":[{"given":"AC","family":"Becher","sequence":"first","affiliation":[{"name":"Centre for Artificial Insemination and Embryo Transfer University of Veterinary Sciences Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Spergser","sequence":"additional","affiliation":[{"name":"Institute of Bacteriology Mycology and Hygiene University of Veterinary Sciences Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Aurich","sequence":"additional","affiliation":[{"name":"Centre for Artificial Insemination and Embryo Transfer University of Veterinary Sciences Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Zottler","sequence":"additional","affiliation":[{"name":"Centre for Artificial Insemination and Embryo Transfer University of Veterinary Sciences Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JE","family":"Aurich","sequence":"additional","affiliation":[{"name":"Division of Obstetrics Gynecology and Andrology University of Veterinary Sciences Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Sch\u00e4fer\u2010Somi","sequence":"additional","affiliation":[{"name":"Centre for Artificial Insemination and Embryo Transfer University of Veterinary Sciences Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00890.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.11.004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.11.021"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"670","DOI":"10.2460\/ajvr.1992.53.05.670","article-title":"Long\u2010term study of aerobic bacteria of the genital tract in stud dogs","volume":"53","author":"Bjurstr\u00f6m L","year":"1992","journal-title":"Am J Vet Res"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.19.1.62-70.1970"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2004.10.002"},{"key":"e_1_2_5_8_1","first-page":"252","article-title":"Experimental bovine genital ureaplasmosis. 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