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Twenty sexually mature females were euthanized at 0 (pre\u2010ovulatory group) and 24, 72 and 96\u00a0h after insemination (pseudopregnancy group). The following lectin\u2010binding agents were used:<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rachis hypogaea,<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>eanut (<jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content>) to label galactosyl (\u03b2\u20101,3)N\u2010 acetyl\u2010galactosamine, Dolichos biflorus Agglutinin (<jats:styled-content style=\"fixed-case\">DBA<\/jats:styled-content>) to label galactosyl (\u03b2\u20101,4)<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010 acetyl\u2010galactosamine,<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ens curinaris (<jats:styled-content style=\"fixed-case\">LCA<\/jats:styled-content>) to label \u03b1\u2010\u2010mannose, \u03b1\u2010<jats:sc>d<\/jats:sc>\u2010glucose and<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>isum sativum agglutinin (<jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content>) to label \u03b1\u2010<jats:sc>d<\/jats:sc>\u2010mannose, \u03b1\u2010<jats:sc>d<\/jats:sc>\u2010glucose. Blood was collected by cardiac puncture, and direct enzyme immunoassay technique was used to measure progesterone concentration. A significant increase in total plasma progesterone concentrations was detected at 96\u00a0h post\u2010ovulation when compared with 0, 24 and 72\u00a0h post\u2010ovulation (2.9\u00a0\u00b1\u00a00.5 vs 0.5\u00a0\u00b1\u00a00.15, 1.6\u00a0\u00b1\u00a00.5 and 1.5\u00a0\u00b1\u00a00.4\u00a0ng\/ml, at 96\u00a0h vs 0, 24 and 72\u00a0h post\u2010ovulation, respectively). No differences between pre\u2010ovulatory and pseudopregnant females were observed for glycoprotein localization in isthmus. In contrast, in the endometrium, differences in the glycoprotein detection between pre\u2010ovulatory and pseudopregnant stages were detected.<jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content>to label galactosyl (\u03b2\u20101,3)N\u2010 acetyl\u2010galactosamine was not detected at the pre\u2010ovulatory stage, but its presence was detected at 24\u00a0h after ovulation. Both<jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">LCA<\/jats:styled-content>to label \u03b1\u2010<jats:sc>d<\/jats:sc>\u2010mannose, \u03b1\u2010<jats:sc>d<\/jats:sc>\u2010glucose were only detected at 72\u00a0h after ovulation.<jats:styled-content style=\"fixed-case\">DBA<\/jats:styled-content>detection was similar for all stages of the reproductive cycle. Therefore, N\u2010acetyl\u2010galactosamine secreted from isthmus could be involved in the formation of the embryonic mucin coat.<jats:sc>d<\/jats:sc>\u2010galactose (<jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content>),<jats:sc>d<\/jats:sc>\u2010glucose and<jats:sc>d<\/jats:sc>\u2010mannose (<jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">LCA<\/jats:styled-content>) might be crucial for the implantation period.<\/jats:p>","DOI":"10.1111\/rda.12195","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T07:48:25Z","timestamp":1371109705000},"page":"967-973","source":"Crossref","is-referenced-by-count":2,"title":["Detection of Glycosylated Proteins in Rabbit Oviductal Isthmus and Uterine Endometrium During Early Embryo Development"],"prefix":"10.1111","volume":"48","author":[{"given":"F","family":"Marco\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Laboratory 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