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The aim of this study was to investigate whether the X\/Y ratio of equine spermatozoa is affected by short\u2010term storage and the <jats:italic>swim\u2010up<\/jats:italic> procedure. We used a standard protocol, for short\u2010term storage (0, 24 and 48\u00a0h at 5\u00b0C) of stallion semen diluted in the commercial extender EquiPro\u2122 (Minit\u00fcb GmbH, Tiefenbach, Germany). After each set\u2010up storage period, the motile fraction of sperm cells was selected by the <jats:italic>swim\u2010up<\/jats:italic> method. The X\/Y ratio was evaluated by fluorescence <jats:italic>in situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) in the fresh, non\u2010selected sperm, and in motile spermatozoa selected after each of the storage periods. Molecular probes for the equine chromosomes X and Y were used. The X\/Y ratio in all sperm samples analysed in this study (fresh and stored) was not different from the theoretical 1\u00a0:\u00a01 value. The incidence of chromosomally abnormal sperm cells in the fresh (0.28%) and motile (0.13%) sperm samples was not significantly different. The two approaches (sperm storage up to 48\u00a0h and the <jats:italic>swim\u2010up<\/jats:italic> procedure) applied to this study did not affect the X\/Y ratio in the motile fraction of equine spermatozoa. This finding does not conform to phenomena described for human and cattle. For this reason, the finding may imply species\u2010related differences.<\/jats:p>","DOI":"10.1111\/rda.12223","type":"journal-article","created":{"date-parts":[[2013,8,7]],"date-time":"2013-08-07T06:56:24Z","timestamp":1375858584000},"page":"52-58","source":"Crossref","is-referenced-by-count":4,"title":["Short\u2010Term Storage and Swim\u2010Up Selection Do Not Affect the X\/Y Ratio in Equine Spermatozoa"],"prefix":"10.1111","volume":"49","author":[{"given":"M","family":"Orsztynowicz","sequence":"first","affiliation":[{"name":"Department of Genetics and Animal Breeding Poznan University of Life Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Pawlak","sequence":"additional","affiliation":[{"name":"Department of Genetics and Animal Breeding Poznan University of Life Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Kociucka","sequence":"additional","affiliation":[{"name":"Department 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