{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:45:00Z","timestamp":1783694700854,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T00:00:00Z","timestamp":1384992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The objective of the present study was to investigate the influence of different sucrose\u2010based extenders on the motility, morphology, viability and acrosomal integrity of epididymal cat spermatozoa cryopreserved by ultra\u2010rapid freezing method. Nine cats were castrated, and collected semen was diluted 1\u00a0:\u00a01 with Dulbecco`s phosphate\u2010buffered saline\u2010<jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>1%\u2010based extender supplemented with different sucrose concentrations (0, 0.25, 0.4 and 0.6\u00a0<jats:sc>m<\/jats:sc>). After ultra\u2010rapid freezing, samples were thawed and sperm motility, morphology, viability and acrosome status were assessed. At thawing, the number of progressively motile (p\u00a0&lt;\u00a00.01) and morphologically normal (p\u00a0&lt;\u00a00.01) sperm was higher in the sucrose\u2010supplemented groups than in the sucrose\u2010free group. Viability of spermatozoa cryopreserved without sucrose was significantly reduced. In extender supplemented with 0.4\u00a0<jats:sc>m<\/jats:sc>sucrose, spermatozoa viability showed higher values (57.0\u00a0\u00b1\u00a04.7; p\u00a0&lt;\u00a00.01). No significant differences were detected among groups for sperm acrosome integrity. Results support that cat sperm survive after ultra\u2010rapid freezing using sucrose as a cryoprotectant, and the best results were achieved when 0.4\u00a0<jats:sc>m<\/jats:sc>of sucrose was used. This is the first report on sperm ultra\u2010rapid freezing of cat sperm and further studies on extenders, sperm management or cryovials should be carried out to improve sperm cryosurvival.<\/jats:p>","DOI":"10.1111\/rda.12253","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T08:30:46Z","timestamp":1385022646000},"source":"Crossref","is-referenced-by-count":22,"title":["Impact of Ultra\u2010Rapid Freezing on the Motility, Morphology, Viability and Acrosome Integrity of Epididymal Cat Sperm Diluted in Sucrose\u2010Based Extenders"],"prefix":"10.1111","volume":"49","author":[{"given":"G","family":"Vizuete","sequence":"first","affiliation":[{"name":"Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EI","family":"Ag\u00fcera","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Physiology and Immunology University of Cordoba Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CC","family":"P\u00e9rez\u2010Mar\u00edn","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1274\/jmor.28.47"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1002\/j.1939-4640.1990.tb03229.x","article-title":"Teratospermic and normospermic domestic cats: ejaculate traits, pituitary\u2010gonadal hormones, and improvement of spermatozoal motility and morphology after swim\u2010up processing","volume":"11","author":"Howard JG","year":"1990","journal-title":"J Androl"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61710-5"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.028811"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0463"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12142"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.12.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.247.3.C125"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2011.12.002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.135"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.11.008"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3923\/jms.2009.30.35"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2010.01054.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2011.01267.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511730207.005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.024"},{"key":"e_1_2_7_19_1","first-page":"113","volume-title":"Deep Freezing of Boar Sperm","author":"Watson P","year":"1985"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12253","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.12253","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T04:54:09Z","timestamp":1716180849000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,21]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/rda.12253"],"URL":"https:\/\/doi.org\/10.1111\/rda.12253","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,21]]}}}