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At 42\u00a0weeks of age, 15 rams were subjected to semen collection and, 1\u00a0week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2\u2010D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>. Total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was isolated from testis, epididymis and vesicular glands and subjected to <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. At slaughter, scrotal circumference and testicular weight were 27.5\u00a0\u00b1\u00a00.5\u00a0cm and 109.5\u00a0\u00b1\u00a06.0\u00a0g, respectively. Seminiferous tubule (ST) diameter was 188.3\u00a0\u00b1\u00a04.0\u00a0<jats:italic>\u03bc<\/jats:italic>m and each testis contained 1.9\u00a0\u00b1\u00a00.1 Sertoli cells (\u00d710<jats:sup>9<\/jats:sup>). Each Sertoli cell supported 0.1\u00a0\u00b1\u00a00.01 A spermatogonia, 3.0\u00a0\u00b1\u00a00.2 pachytene spermatocytes and 7.7\u00a0\u00b1\u00a00.5 round spermatids\/tubule cross section. Daily sperm production reached 5.6\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0cells\/g of testis parenchyma. Testis size appeared as indicative of <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content> diameter and associated with epididymal measurements, as well as with the population of round spermatids and Sertoli cells\/testis. Rams with heavier testes had greater daily sperm production and more Sertoli cells\/testis. We detected 90.9\u00a0\u00b1\u00a09.6 spots per 2\u2010D gel of seminal plasma. Major seminal proteins were identified as ram seminal vesicle proteins at 14 and 22\u00a0kDa, representing 16.2% and 12.8% of the total intensity of valid spots in the gels, respectively. Expression of both genes was greater in the vesicular glands as compared to testis and epididymis. Pixel intensity for those proteins in the 2\u2010D gels was significantly correlated with seminal vesicle weight. This is the first description of the basic reproductive aspects of <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>orada <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ova rams, including protein profiles of their seminal plasma. These findings will allow a better understanding of their reproductive physiology.<\/jats:p>","DOI":"10.1111\/rda.12288","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T04:33:34Z","timestamp":1397104414000},"page":"409-419","source":"Crossref","is-referenced-by-count":11,"title":["Parameters of the Reproductive Tract, Spermatogenesis, Daily Sperm Production and Major Seminal Plasma Proteins of Tropically Adapted Morada Nova Rams"],"prefix":"10.1111","volume":"49","author":[{"given":"FML","family":"Sousa","sequence":"first","affiliation":[{"name":"Department of Animal Science Federal University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CH","family":"Lobo","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Cear\u00e1 Fortaleza 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