{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:12:53Z","timestamp":1767849173450,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,3,11]],"date-time":"2014-03-11T00:00:00Z","timestamp":1394496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The mammalian sperm membrane undergoes cholesterol efflux during maturation and fertilization. Although <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\u2010binding cassette (<jats:styled-content style=\"fixed-case\">ABC<\/jats:styled-content>) transporters are known to transport cholesterol through cell membranes in other organs, their presence in canine testis, epididymis and sperm has not been proven to date. Hence, the aim of the present study was to localize the <jats:styled-content style=\"fixed-case\">ABC<\/jats:styled-content> transporters <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">ABCG<\/jats:styled-content>1 in canine testicular and epididymidal tissue as well as in spermatozoa membranes. To this end, semen samples from 12 dogs as well as testicles and epididymides of four young and healthy dogs were prepared for immunohistochemistry, respectively. Capacitation and acrosome reaction (<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>) were induced in aliquots of the semen samples before immunostaining to assess changes in the expression of <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">ABCG<\/jats:styled-content>1. Evaluation by confocal microscopy revealed the presence of both <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">ABCG<\/jats:styled-content>1 in canine testicles and of <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 in the epididymides. In spermatozoa, only <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 immunoreactivity was detected, mainly in the region of the acrosome and midpiece. After induction of capacitation, <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 signal persisted in the acrosome but disappeared after <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>, indicating a loss of <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 with the loss of the acrosome. We conclude that <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">ABCG<\/jats:styled-content>1 are expressed in canine testis, whereas only <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 is expressed in epididymis and spermatozoa membrane, both transporters probably contributing to the regulation of membrane cholesterol content.<\/jats:p>","DOI":"10.1111\/rda.12294","type":"journal-article","created":{"date-parts":[[2014,3,11]],"date-time":"2014-03-11T05:52:54Z","timestamp":1394517174000},"page":"441-447","source":"Crossref","is-referenced-by-count":17,"title":["Immunolocalization of the Cholesterol Transporters <scp>ABCA<\/scp>1 and <scp>ABCG<\/scp>1 in Canine Reproductive Tract Tissues and Spermatozoa"],"prefix":"10.1111","volume":"49","author":[{"given":"N","family":"Palme","sequence":"first","affiliation":[{"name":"Veterinary Clinic G\u00e4nserndorf Vienna 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