{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:28:52Z","timestamp":1761863332650},"reference-count":53,"publisher":"Wiley","issue":"s2","license":[{"start":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T00:00:00Z","timestamp":1403222400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Canine sperm transport, distribution, storage and detachment is a complex, dynamic and highly regulated process. Transport of sperm within the bitch's reproductive tract is rapid and is influenced by the method of semen deposition (natural mating or artificial insemination) and by the timing of breeding in relation to the day of ovulation. The fertile lifespan of spermatozoa in the reproductive tract of the bitch is considerably longer than in most other domestic species, and the main sperm reservoirs appear to be the uterine crypts and the distal part of the uterotubal junction, where spermatozoa attach by their heads to uterine epithelium. While several<jats:italic>in vitro<\/jats:italic>studies demonstrated prolonged motility and viability of canine spermatozoa after coincubation with uterine tube explants, spermatozoal storage has not been documented in the canine uterine tube isthmus or ampulla<jats:italic>in vivo<\/jats:italic>. Several factors, including exposure to progesterone, solubilized zona pellucida proteins and post\u2010ovulation uterine tube fluid, appear to trigger membrane events resulting in capacitation\u2010like changes with subsequent motility pattern changes (transitional and hyperactivated) that are associated with sperm detachment. After mating or insemination, a normal low\u2010magnitude post\u2010mating uterine inflammatory response occurs, evidenced by an influx of polymorphonuclear neutrophils (<jats:styled-content style=\"fixed-case\">PMN<\/jats:styled-content>s), increased uterine contractions and an increased uterine artery blood flow. Recently, it was also shown that normal dogs with cystic endometrial hyperplasia develop a more significant endometritis, show fewer mating\u2010induced uterine contractions, a decreased ability of spermatozoa to bind to uterine explants<jats:italic>in vitro<\/jats:italic>and a slower uterine clearance after mating.<\/jats:p>","DOI":"10.1111\/rda.12299","type":"journal-article","created":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T12:45:35Z","timestamp":1403268335000},"page":"2-7","source":"Crossref","is-referenced-by-count":17,"title":["Current Knowledge on the Transport and Fate of Spermatozoa in the Reproductive Tract of the Bitch"],"prefix":"10.1111","volume":"49","author":[{"given":"T","family":"Rijsselaere","sequence":"first","affiliation":[{"name":"Department of Reproduction Obstetrics and Herd Health Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GCW","family":"England","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science University of Nottingham Leicestershire UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SL","family":"Freeman","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science University of Nottingham Leicestershire UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Maes","sequence":"additional","affiliation":[{"name":"Department of Reproduction Obstetrics and Herd Health Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Van Soom","sequence":"additional","affiliation":[{"name":"Department of Reproduction Obstetrics and Herd Health Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1114"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2011.08.026"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod17.4.604"},{"key":"e_1_2_11_5_1","first-page":"3","article-title":"Biology and endocrinology of ovulation, pregnancy and parturition in the dog","volume":"39","author":"Concannon P","year":"1989","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.6.1395"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0130051"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1995.tb00574.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.2460\/ajvr.1996.57.11.1635","article-title":"Role of carbohydrates in the attachment of equine spermatozoa to uterine tubal epithelial cells in vitro","volume":"57","author":"Dobrinski I","year":"1996","journal-title":"Am J Vet Res"},{"key":"e_1_2_11_10_1","first-page":"1","article-title":"Natural history of mammalian spermatozoa in the female reproductive tract","volume":"14","author":"Drobnis EZ","year":"1992","journal-title":"Oxf Rev Reprod Biol"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.136.21.542"},{"key":"e_1_2_11_12_1","first-page":"325","article-title":"Survival of dog spermatozoa within the reproductive tract of the bitch","volume":"38","author":"England GCW","year":"2003","journal-title":"Reprod Domest Anim"},{"key":"e_1_2_11_13_1","first-page":"57","article-title":"Transportation and interaction of dog spermatozoa within the reproductive tract of the bitch: comparative aspects","volume":"3","author":"England GCW","year":"1998","journal-title":"Cent Reprod Biol"},{"key":"e_1_2_11_14_1","first-page":"624","article-title":"Variability of the time of calculated luteinising hormone release in 218 canine pregnancies","volume":"125","author":"England GCW","year":"1989","journal-title":"Vet Rec"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.025"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.04.031"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.07.009"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12019"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.12.003"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.04.027"},{"key":"e_1_2_11_21_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1152\/ajplegacy.1933.105.2.287","article-title":"The transport of spermatozoa in the dog","volume":"105","author":"Evans EI","year":"1933","journal-title":"Am J Physiol"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(81)90043-4"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057006"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.03.024"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.06.026"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01493.x"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1220305"},{"key":"e_1_2_11_28_1","first-page":"82","article-title":"Ovarian endocrine control of sperm progression in the fallopian tubes","volume":"17","author":"Hunter RHF","year":"1995","journal-title":"Oxf Rev Reprod Biol"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402660212"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19801013"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(91)90020-T"},{"key":"e_1_2_11_32_1","unstructured":"Iguer\u2010ouadaM 2000:Oestrus Cycle Stage dependent Effects of Plasma and Vaginal Fluid on Dog Semen Motility Parameters. In: Medically Assisted Procreation in Canine Species: Analysis and 4\u00b0C Preservation of Semen. PhD thesis Faculty of Veterinary Medicine Li\u00e8ge University pp.187\u2013202."},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-54-49"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00135-X"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod56.5.1198"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(96)01566-7"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(96)01631-4"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod19.1.101"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00329-0"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.09.011"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0800445"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00500"},{"key":"e_1_2_11_43_1","unstructured":"RijsselaereT 2004. In:New Techniques for Canine Semen Assessment and Characterization of the Sperm Reservoir in the Bitch. PhD thesis Faculty of Veterinary Medicine Ghent University pp189\u2013212."},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00273"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1071\/RDv17n2Ab4"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00130-5"},{"key":"e_1_2_11_47_1","first-page":"74","volume-title":"The Biology of Spermatozoa","author":"Settlage DSF","year":"1975"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod37.1.225"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.00346.x"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod56.2.447"},{"key":"e_1_2_11_51_1","first-page":"269","article-title":"Gamete physiology and timing of ovulation and fertilization in dogs","volume":"39","author":"Tsutsui T","year":"1989","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms1939.51.560"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21300"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod18.4.561"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12299","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T22:41:40Z","timestamp":1716849700000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":53,"journal-issue":{"issue":"s2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/rda.12299"],"URL":"https:\/\/doi.org\/10.1111\/rda.12299","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]}}}