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The present study investigated the role of gonadotrophins in cervical function by examining the expression of follicle\u2010stimulating hormone receptor (FSHR<\/jats:styled-content>) and luteinizing hormone receptor (LHR<\/jats:styled-content>) and their mRNA<\/jats:styled-content>s following intracervical treatment with either FSH<\/jats:styled-content> or LH<\/jats:styled-content>. Eighteen ewes were assigned to four groups, and they were then treated with progestagen sponges and PMSG<\/jats:styled-content> to synchronize their oestrous cycles. Intracervical treatments were given 24\u00a0h after sponge removal as follows: G<\/jats:styled-content>roup 1: FSH<\/jats:styled-content> 2\u00a0mg; G<\/jats:styled-content>roup 2: LH<\/jats:styled-content> 2\u00a0mg; G<\/jats:styled-content>roup 3: V<\/jats:styled-content>ehicle and G<\/jats:styled-content>roup 4: C<\/jats:styled-content>ontrol. Cervices were collected 54\u00a0h after sponge removal and then divided into three regions. The expression of FSHR<\/jats:styled-content> and LHR<\/jats:styled-content> was determined by immunohistochemistry and FSHR<\/jats:styled-content> mRNA<\/jats:styled-content> and LH<\/jats:styled-content> mRNA<\/jats:styled-content> by in situ<\/jats:italic> hybridization. The expression of LHR<\/jats:styled-content>, FSHR<\/jats:styled-content> and their respective mRNA<\/jats:styled-content>s was compared in six tissue layers (luminal epithelium, subepithelial stroma, circular, longitudinal and transverse muscle and serosa) and in three cervical regions (vaginal, mid and uterine). The results showed that FSH<\/jats:styled-content> increased transcription of the FSHR<\/jats:styled-content> gene and the levels of its receptor, but only in subepithelial stroma of the cervix. FSH<\/jats:styled-content> also increased the levels of LHR<\/jats:styled-content> in the cervix, but only in the muscle layers. LH<\/jats:styled-content> had no effect on the levels of FSHR<\/jats:styled-content> despite the fact that it did increase the level of transcription of the FSHR<\/jats:styled-content> gene and LH<\/jats:styled-content> also increased the levels of its own receptor in the cervix, but only in the muscle layers, and this action was independent of increased levels of transcription of the LHR<\/jats:styled-content> gene. These findings suggest multiple levels of regulation of cervical LH<\/jats:styled-content> and FSH<\/jats:styled-content> receptors and that the gonadotrophins may have a role in relaxation of the cervix during oestrus by regulating their own receptors.<\/jats:p>","DOI":"10.1111\/rda.12312","type":"journal-article","created":{"date-parts":[[2014,4,23]],"date-time":"2014-04-23T05:53:01Z","timestamp":1398232381000},"page":"568-575","source":"Crossref","is-referenced-by-count":8,"title":["The Effect of the Intracervical Application of Follicle\u2010Stimulating Hormone or Luteinizing Hormone on the Pattern of Expression of Gonadotrophin Receptors in the Cervix of Non\u2010Pregnant Ewes"],"prefix":"10.1111","volume":"49","author":[{"given":"S","family":"Leethongdee","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Sciences Mahasarakham University Amphur Muang Mahasarakham 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