{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T02:56:49Z","timestamp":1769309809306,"version":"3.49.0"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,4,16]],"date-time":"2014-04-16T00:00:00Z","timestamp":1397606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>A 1\u2010year\u2010old Shih Tzu dog was presented for examination because of abnormal external genitalia. A residual penis with a prepuce was located in a position typical of a male. The dog had no palpable testicles or scrotum. The ultrasound examination revealed the presence of the prostate, but the gonads remained undetectable. Cytogenetic analysis performed on chromosome preparations obtained from lymphocyte culture showed two cell lines \u2013 78,<jats:styled-content style=\"fixed-case\">XX<\/jats:styled-content> and 78,<jats:styled-content style=\"fixed-case\">XY<\/jats:styled-content>. Molecular analysis of 14 polymorphic microsatellite markers allowed us to distinguish leucocyte chimerism from whole body chimerism. The presence of 3 or 4 alleles was confirmed in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> isolated from blood, while in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> isolated from hair follicles only 1 or 2 alleles were detected. The case was classified as leucocyte 78,<jats:styled-content style=\"fixed-case\">XX<\/jats:styled-content>\/78,<jats:styled-content style=\"fixed-case\">XY<\/jats:styled-content> chimerism. Our study showed that <jats:styled-content style=\"fixed-case\">XX<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">XY<\/jats:styled-content> leucocyte chimerism might be associated with disorder of sexual development in dogs. Furthermore, it is emphasized that the use of cytogenetic study, in combination with analysis of polymorphic markers in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> isolated from different somatic cells, facilitates distinguishing between leucocyte and whole body chimerism.<\/jats:p>","DOI":"10.1111\/rda.12318","type":"journal-article","created":{"date-parts":[[2014,4,16]],"date-time":"2014-04-16T05:58:45Z","timestamp":1397627925000},"source":"Crossref","is-referenced-by-count":12,"title":["A Case of Leucocyte Chimerism (78,<scp>XX<\/scp>\/78,<scp>XY<\/scp>) in a Dog with a Disorder of Sexual Development"],"prefix":"10.1111","volume":"49","author":[{"given":"I","family":"Szczerbal","sequence":"first","affiliation":[{"name":"Department of Genetics and Animal Breeding Poznan University of Life Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Nowacka\u2010Woszuk","sequence":"additional","affiliation":[{"name":"Department of Genetics and Animal Breeding Poznan University of Life Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Nizanski","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals Wroclaw University of Environmental and Life Sciences Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Salamon","sequence":"additional","affiliation":[{"name":"Department of Genetics and Animal Breeding Poznan University of Life Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Ochota","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals Wroclaw University of Environmental and Life Sciences Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Dzimira","sequence":"additional","affiliation":[{"name":"Department of Pathology Wroclaw University of Environmental and Life Sciences Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Atamaniuk","sequence":"additional","affiliation":[{"name":"Department and Clinic of Surgery Wroclaw University of Environmental and Life Sciences Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Switonski","sequence":"additional","affiliation":[{"name":"Department of Genetics and Animal Breeding Poznan University of Life Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,16]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"BeccagliaM RoncheseM GriecoV ParmaP LuvoniGC 2012:XX\/XY chimaerism\/mosaicism in a phenotypically female Wirehaired Pointing Griffon dog. 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