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In total, 108 semen samples were obtained from artificial insemination centres, and genomic<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>of<jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0gondii<\/jats:italic>was detected in 24 of 108 (22.2%). The prevalence of antibodies anti\u2010<jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>oxoplasma gondii<\/jats:italic>among sheep on rural properties was 9.2% (10\/109), and 100% of the semen samples of these animals were positive in the<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>for<jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0gondii<\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. The molecular identity was confirmed through sequencing, which indicated 99.9% similarity with the<jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0gondii<\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>sequences stored in the<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>en<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>ank. This study reports the first occurrence of<jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0gondii<\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>in the semen of rams, which came from artificial insemination centres in<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>razil, as well as the occurrence of<jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0gondii<\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>in the fresh semen of naturally infected rams in the northeast of<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>razil.<\/jats:p>","DOI":"10.1111\/rda.12361","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T10:39:29Z","timestamp":1407753569000},"page":"753-755","source":"Crossref","is-referenced-by-count":15,"title":["Detection of<i><scp>T<\/scp>oxoplasma 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