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Ejaculates (n\u00a0=\u00a025) from five donkeys were diluted in<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MEDIUM<\/jats:styled-content>with 0, 1, 2 or 3\u00a0mg of<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>\/120\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>spermatozoa. Semen was frozen, and thawed samples were evaluated by computer\u2010assisted sperm analyser system (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>), supravital test, hyposmotic swelling test and fluorescent dyes to assess the integrity of sperm membranes. Mares (n\u00a0=\u00a060) were inseminated with frozen\u2010thawed semen treated with the doses of 0 or 1\u00a0mg<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>. Percentages of sperm with progressive motility and with functional plasma membrane were greater (p\u00a0&lt;\u00a00.05) in the<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>\u2010treated groups than in the control. Percentages of intact plasma membrane and intact plasma membrane and acrosome detected by fluorescent dyes were also greater (p\u00a0&lt;\u00a00.05) in<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>\u2010treated groups. Although no difference (p\u00a0&gt;\u00a00.05) in conception rates was detected between groups (control, 3\/30, 10%;<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>\u2010treated, 1\/30, 3.3%), fertility was low for artificial insemination programs in mares. Therefore, we firstly demonstrated that frozen semen treated with<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>in<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MEDIA<\/jats:styled-content>extender before freezing improves the<jats:italic>in vitro<\/jats:italic>sperm viability, but semen treated or not with<jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content>in<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MEDIUM<\/jats:styled-content>extender results in a very low conception rate in mares inseminated with thawed donkey semen.<\/jats:p>","DOI":"10.1111\/rda.12379","type":"journal-article","created":{"date-parts":[[2014,8,14]],"date-time":"2014-08-14T14:17:11Z","timestamp":1408025831000},"page":"845-850","source":"Crossref","is-referenced-by-count":18,"title":["Use of Cholesterol\u2010Loaded Cyclodextrin in Donkey Semen Cryopreservation Improves Sperm Viability but Results in Low Fertility in Mares"],"prefix":"10.1111","volume":"49","author":[{"given":"RR","family":"Oliveira","sequence":"first","affiliation":[{"name":"Department of Animal Science Federal University of Vi\u00e7osa Vi\u00e7osa MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DM","family":"Rates","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Vi\u00e7osa Vi\u00e7osa MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Pugliesi","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Vi\u00e7osa Vi\u00e7osa MG Brazil"},{"name":"Department of Animal Reproduction Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PG","family":"Ker","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Vi\u00e7osa Vi\u00e7osa MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RP","family":"Arruda","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EA","family":"Moraes","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Vale do S\u00e3o Francisco Petrolina PE Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GR","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Vi\u00e7osa Vi\u00e7osa MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.04.026"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2008.12.007"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.2000.tb03268.x","article-title":"Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon","volume":"21","author":"Bailey JL","year":"2000","journal-title":"J Androl"},{"key":"e_1_2_5_5_1","first-page":"133","article-title":"Effects of cryopreservation on the acrosomal status of stallion spermatozoa","volume":"56","author":"Bedford SJ","year":"2000","journal-title":"J Reprod Fertil"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2010.00130.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00810.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1210395"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1208\/pt060243"},{"key":"e_1_2_5_10_1","first-page":"127","article-title":"Effect of cholesterol on the motility e plasma membrane integrity of frozen equine spermatozoa after thawing","volume":"56","author":"Combes GB","year":"2000","journal-title":"J Reprod Fertil"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1997.tb01953.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(77)90008-6"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9731409"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.056"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1956.tb00443.x"},{"key":"e_1_2_5_16_1","volume-title":"CBRA \u2013 Brazillian College of Animal Reproduction. 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