{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:26:41Z","timestamp":1773404801523,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T00:00:00Z","timestamp":1411516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001862","name":"Formas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The objective of this study was to evaluate bull sperm kinematics after centrifugation through a single layer of a colloid [Single Layer Centrifugation (<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>)]. Ejaculates from 20 bulls were extended and stored at 4\u20136\u00b0C for 24\u00a0h during transport to the laboratory for<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>through Androcoll\u2010B, followed by measurement of sperm kinematics in all samples. Total motility (86% and 88% for uncentrifuged and<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>samples, respectively) and progressive motility (84% for both the groups) were similar (p\u00a0&gt;\u00a00.05). In contrast, straightness (STR) (0.65 vs 0.69), linearity (LIN) (0.32 vs 0.35) and beat cross frequency (BCF) (22.3 vs 23.6\u00a0Hz) were significantly higher in the<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>\u2010selected samples than in the uncentrifuged samples, whereas velocity of the average path (VAP) (95 vs 90\u00a0\u03bcm\/s), curvilinear velocity (VCL) (192 vs 180\u00a0\u03bcm\/s), amplitude of lateral head deviation (ALH) (7\u00a0\u03bcm vs 6.5\u00a0\u03bcm) and hypermotility (49% vs 38%) were significantly decreased. The kinematics of the samples with the poorest motility was improved most by<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>. In conclusion, even though<jats:styled-content style=\"fixed-case\">SLC<\/jats:styled-content>had no direct effect on total and progressive motility, it appeared to have a positive influence on several other kinematic parameters that may be important for fertilization after artificial insemination.<\/jats:p>","DOI":"10.1111\/rda.12412","type":"journal-article","created":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T05:32:30Z","timestamp":1411536750000},"page":"954-956","source":"Crossref","is-referenced-by-count":9,"title":["Changes in Bull Sperm Kinematics after Single Layer Centrifugation"],"prefix":"10.1111","volume":"49","author":[{"given":"Y","family":"Yulnawati","sequence":"first","affiliation":[{"name":"Division of Reproduction Department of Clinical Sciences Swedish University of Agricultural Sciences (SLU) Uppsala Sweden"},{"name":"Research Center for Biotechnology Indonesian Institute of Sciences (LIPI) Cibinong Bogor Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MC","family":"Abraham","sequence":"additional","affiliation":[{"name":"Division of Reproduction Department of Clinical Sciences Swedish University of Agricultural Sciences (SLU) Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Laskowski","sequence":"additional","affiliation":[{"name":"Division of Reproduction Department of Clinical Sciences Swedish University of Agricultural Sciences (SLU) Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Johannisson","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Physiology and Biochemistry Swedish University of Agricultural Sciences (SLU) Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JM","family":"Morrell","sequence":"additional","affiliation":[{"name":"Division of Reproduction Department of Clinical Sciences Swedish University of Agricultural Sciences (SLU) Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01159-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.049"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1439-0531.2007.00853.x","article-title":"Effects of filtration of semen doses from subfertile boars through neuter Sephadex columns","volume":"43","author":"Bussallou E","year":"2008","journal-title":"Reprod Domest Anim"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013-7607"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00682.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2011.00489.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.06.015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2012.05.069"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01866.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.05.015"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/3.5.403"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01081.x"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12412","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12412","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T21:57:02Z","timestamp":1717365422000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,24]]},"references-count":12,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/rda.12412"],"URL":"https:\/\/doi.org\/10.1111\/rda.12412","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,24]]}}}