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Addition of autologous seminal plasma to frozen\u2010thawed semen appeared to improve pregnancy rates. The aims of this study were to evaluate (1) sperm motility and plasma membrane integrity after thawing (T0) and after one and 2\u00a0h (T1 and T2) of post\u2010thaw incubation in either 0% (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>0) or 70% (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>70) autologous seminal plasma and (2) sperm motility, plasma membrane integrity and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> quality (%<jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\u2010\u03b1t) after thawing (T0) and after 2 and 4\u00a0h (T2 and T4) of post\u2010thaw incubation in either 0% (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>0), 5% (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>5) or 20% (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>20) homologous seminal plasma. In experiment 1, seminal plasma decreased total and progressive sperm motility and plasma membrane intact spermatozoa immediately after dilution and at all following time points (p\u00a0&lt;\u00a00.05). In experiment 2, total and progressive motility did not differ between treatments immediately after dilution and between <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>0 and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>5 at T2, while they were lower in both <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>5 and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>20 than in <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>0 at T4. Plasma membrane intact sperm cells did not differ between <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>0 and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>5 and were lower in <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>20 at all time points. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> quality was not affected by treatment immediately after dilution and was significantly worse for <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>20 after 4\u00a0h of incubation (p\u00a0&lt;\u00a00.05). The post\u2010thaw addition of seminal plasma at the tested concentrations did not improve donkey frozen semen characteristics <jats:italic>in vitro<\/jats:italic> over time.<\/jats:p>","DOI":"10.1111\/rda.12419","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T04:58:16Z","timestamp":1411621096000},"page":"989-994","source":"Crossref","is-referenced-by-count":18,"title":["Effect of Post\u2010Thaw Addition of Seminal Plasma on Motility, Viability and Chromatin Integrity of Cryopreserved Donkey Jack (<i>Equus asinus<\/i>) Spermatozoa"],"prefix":"10.1111","volume":"49","author":[{"given":"C","family":"Sabatini","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Mari","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Mislei","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CC","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences College of Veterinary Medicine and Biosciences, Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Panzani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Camillo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Rota","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,25]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"AlvarezAL SerresC CrespoF SantiagoI MateosE Gomez\u2010CuetaraC 2004:Natural service and artificial insemination with cooled semen of Zamorano\u2010Leones donkey. 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