{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T06:44:13Z","timestamp":1696229053437},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,15]],"date-time":"2014-11-15T00:00:00Z","timestamp":1416009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of this study was to investigate how acute insulin\u2010induced hypoglycaemia (IIH) alters the activity of cells containing oestradiol receptor <jats:italic>\u03b1<\/jats:italic> (ER<jats:italic>\u03b1<\/jats:italic>) or somatostatin (SST) in the arcuate nucleus (ARC) and ventromedial nucleus (VMN), and ER<jats:italic>\u03b1<\/jats:italic> cells in the medial preoptic area (m<jats:styled-content style=\"fixed-case\">POA<\/jats:styled-content>) of intact ewes. Follicular phases were synchronized with progesterone vaginal pessaries. Control animals were killed at 0\u00a0h or 31\u00a0h (n\u00a0=\u00a05 and 6, respectively) after progesterone withdrawal (PW; time zero). At 28\u00a0h, five other animals received insulin (INS; 4\u00a0iu\/kg) and were subsequently killed at 31\u00a0h. Hypothalamic sections were immunostained for ER<jats:italic>\u03b1<\/jats:italic> or SST each with c\u2010Fos, a marker of neuronal transcriptional activation. Insulin did not alter the percentage of activated ER<jats:italic>\u03b1<\/jats:italic> cells in the ARC; however, it appeared visually that two insulin\u2010treated animals (INS responders, with no LH surge) had an increase in the VMN (from 32 to 78%) and a decrease in the m<jats:styled-content style=\"fixed-case\">POA<\/jats:styled-content> (from 40 to 12%) compared to no increase in the two INS non\u2010responders (with an LH surge). The percentage of activated SST cells in the ARC was greater in all four insulin\u2010treated animals (from 10 to 60%), whereas it was visually estimated that activated SST cells in the VMN increased only in the two insulin responders (from 10 to 70%). From these results, we suggest that IIH stimulates SST activation in the ARC as part of the glucose\u2010sensing mechanism but ER<jats:italic>\u03b1<\/jats:italic> activation is unaffected in this region. We present evidence to support a hypothesis that disruption of the GnRH\/LH surge may occur in insulin responders via a mechanism that involves, at least in part, SST cell activation in the VMN along with decreased ER<jats:italic>\u03b1<\/jats:italic> cell activation in the m<jats:styled-content style=\"fixed-case\">POA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/rda.12450","type":"journal-article","created":{"date-parts":[[2014,11,15]],"date-time":"2014-11-15T05:54:15Z","timestamp":1416030855000},"page":"68-75","source":"Crossref","is-referenced-by-count":2,"title":["Co\u2010Expression of c\u2010Fos with Oestradiol Receptor \u03b1 or Somatostatin in the Arcuate Nucleus, Ventromedial Nucleus and Medial Preoptic Area in the Follicular Phase of Intact Ewes: Alteration after Insulin\u2010Induced Hypoglycaemia"],"prefix":"10.1111","volume":"50","author":[{"given":"C","family":"Fergani","sequence":"first","affiliation":[{"name":"School of Veterinary Science University of Liverpool 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