{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:26:03Z","timestamp":1740126363333,"version":"3.37.3"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T00:00:00Z","timestamp":1416528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Cryopreservation of epididymal spermatozoa is often performed after shipping the excised testis\u2013epididymis complexes, under refrigeration, to a specialized laboratory. However, epididymal spermatozoa can be collected immediately after excision of the epididymis and sent extended and refrigerated to a laboratory for cryopreservation. In this experiment, we evaluated the effect of both methods of cold storage bovine epididymal spermatozoa as well as of two different extenders on spermatozoa characteristics after freeze\u2013thawing. For that, spermatozoa collected from the caudae epididymis of 19 bulls were extended and cryopreserved in either AndroMed<jats:sup>\u00ae<\/jats:sup> or a Tris\u2013egg yolk (<jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content>)\u2010based extender. Cryopreservation of sperm cells was performed immediately after castration (Group A, n\u00a0=\u00a09) or after cold storage for 24\u00a0h diluted in the two extenders and (Group B, n\u00a0=\u00a09) and also after cold storage for 24\u00a0h within the whole epididymis (Group C, n\u00a0=\u00a010). Sperm subjective progressive motility (light microscopy), plasma membrane integrity (hypoosmotic swelling test) and sperm viability (eosin\u2013nigrosin) were evaluated. <jats:italic>In vitro<\/jats:italic> fertilization and culture (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>) was performed to assess the blastocyst rate. No differences (p\u00a0&gt;\u00a00.05) were observed on post\u2010thaw sperm parameters between samples from Group A, B and C. <jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content> extended samples presented a higher (p\u00a0&lt;\u00a00.01) percentage of progressive motile and live sperm, than those extended in AndroMed<jats:sup>\u00ae<\/jats:sup>. Blastocyst rate after <jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content> differed only (p\u00a0&lt;\u00a00.05) between the reference group (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content> performed with frozen semen with known <jats:italic>in vitro<\/jats:italic> fertility) and Group A extended in AndroMed<jats:sup>\u00ae<\/jats:sup>. We conclude that when cryopreservation facilities are distant from the collection site, bovine epididymal sperm can be shipped chilled overnight either within the epididymal tail or after dilution without deleterious effect on post\u2010thaw sperm quality. <jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content> extender was more suitable for cold storage and freezing bovine epididymal sperm, than the commercial extender AndroMed\u00ae.<\/jats:p>","DOI":"10.1111\/rda.12454","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T05:35:47Z","timestamp":1416548147000},"page":"97-103","source":"Crossref","is-referenced-by-count":11,"title":["Tris\u2013Egg Yolk\u2013Glycerol (<scp>TEY<\/scp>) Extender Developed for Freezing Dog Semen is a Good Option to Cryopreserve Bovine Epididymal Sperm Cells"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6734-974X","authenticated-orcid":false,"given":"G","family":"Lopes","sequence":"first","affiliation":[{"name":"ICBAS Abel Salazar Institute of Biomedical Science University of Porto Porto Portugal"},{"name":"CECA University of Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Soares","sequence":"additional","affiliation":[{"name":"ICBAS Abel Salazar Institute of Biomedical Science University of Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Ferreira","sequence":"additional","affiliation":[{"name":"ICBAS Abel Salazar Institute of Biomedical Science University of Porto Porto Portugal"},{"name":"CECA University of Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Rocha","sequence":"additional","affiliation":[{"name":"ICBAS Abel Salazar Institute of Biomedical Science University of Porto Porto Portugal"},{"name":"CECA University of Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.07.002"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.04.018"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2000113"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.02.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.2527\/jas1989.6782181x","article-title":"In vitro fertilization and development of in vitro matured bovine follicular oocytes","volume":"67","author":"Goto K","year":"1989","journal-title":"J Anim Sci"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.10.028"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.007"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(68)87257-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00139-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00166-6"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.10.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.01.018"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.12.018"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2012.08.010"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.032"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.1998.tb01370.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120170408"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.03.013"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00248-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.04.011"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.11.003"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.01.012"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12454","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12454","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:56:39Z","timestamp":1696172199000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,21]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/rda.12454"],"URL":"https:\/\/doi.org\/10.1111\/rda.12454","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2014,11,21]]}}}