{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T15:24:42Z","timestamp":1775661882421,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,7]],"date-time":"2015-02-07T00:00:00Z","timestamp":1423267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Contents<\/jats:title>Effects of Equex and glycerol additions and sample dilution step on frozen\u2013thawed epididymal cat spermatozoa were investigated. The epididymal sperm pellets were resuspended in extenders using one\u2010 (groups III<\/jats:styled-content> and IV<\/jats:styled-content>) or two\u2010 (groups I, II<\/jats:styled-content>, V and VI<\/jats:styled-content>) step dilution. For one\u2010step dilution, the pellets were resuspended in plain egg yolk\u2010Tris medium (EYT<\/jats:styled-content>)\u00a0+\u00a05% glycerol with (IV<\/jats:styled-content>)\/without (III<\/jats:styled-content>) 0.5% Equex and cooled (4\u00b0<\/jats:sup>C, 1\u00a0h). For two\u2010step dilution, the pellets were resuspended in EYT<\/jats:styled-content> (I and V) and in EYT<\/jats:styled-content>\u00a0+\u00a03% glycerol (II<\/jats:styled-content> and VI<\/jats:styled-content>), cooled and further diluted with EYT<\/jats:styled-content>\u00a0+\u00a010% glycerol with (I)\/without (V) 1% Equex and with EYT<\/jats:styled-content>\u00a0+\u00a07% glycerol with (II<\/jats:styled-content>)\/without (VI<\/jats:styled-content>) 1% Equex. Immediately after freeze\u2013thawing, no differences (p\u00a0>\u00a00.05) were found in the motility, viability and membrane integrity (HOST<\/jats:styled-content>) among the groups except the lowest HOST<\/jats:styled-content> in IV<\/jats:styled-content> (p\u00a0=\u00a00.005 to p\u00a0=\u00a00.04). The acrosome integrity (FITC<\/jats:styled-content>) in group I was comparable to that in group II<\/jats:styled-content> (p\u00a0>\u00a00.05) and was higher than the rest (p\u00a0<\u00a00.001 to p\u00a0=\u00a00.02). At 2\u00a0h after thawing, the motility, viability and HOST<\/jats:styled-content> were comparable among the groups (p\u00a0>\u00a00.05) except the lower percentages of viability in III<\/jats:styled-content> (p\u00a0=\u00a00.008 to p\u00a0=\u00a00.3) and of HOST<\/jats:styled-content> in IV<\/jats:styled-content> (p\u00a0=\u00a00.005 to p\u00a0=\u00a00.2). Two\u2010step dilutions with Equex (I, II<\/jats:styled-content>) were more beneficial for the FITC<\/jats:styled-content> at 2\u00a0h than without Equex (V) (p\u00a0=\u00a00.005 and p\u00a0=\u00a00.02) and than one\u2010step dilutions (III<\/jats:styled-content>, IV<\/jats:styled-content>) (p\u00a0<\u00a00.001 to p\u00a0=\u00a00.02). In conclusion, epididymal cat sperm quality after freeze\u2013thawing could be improved when Equex was added and two\u2010step dilution was performed during freezing. The extenders prepared for the first step of dilution could be with (3%) or without (0%) glycerol.<\/jats:p>","DOI":"10.1111\/rda.12496","type":"journal-article","created":{"date-parts":[[2015,2,7]],"date-time":"2015-02-07T07:21:03Z","timestamp":1423293663000},"page":"378-385","source":"Crossref","is-referenced-by-count":16,"title":["Determination of Appropriate Cryopreservation Protocols for Epididymal Cat Spermatozoa"],"prefix":"10.1111","volume":"50","author":[{"given":"K","family":"Buranaamnuay","sequence":"first","affiliation":[{"name":"Reproductive Biology Research Group Institute of Molecular Biosciences (MB) Mahidol University Nakhon Pathom Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1988.66112899x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2003.11.003"},{"key":"e_1_2_8_4_1","first-page":"545","article-title":"Freezing of cat semen in straws with different glycerol levels containing Tris extender","volume":"28","author":"Baran A","year":"2004","journal-title":"Turk J Vet Anim Sci"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-008-9081-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(72)90010-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00996.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.12.009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.10.017"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.106.000505"},{"key":"e_1_2_8_11_1","first-page":"297","article-title":"Comparative cryopreservation and capacitation of spermatozoa from epididymides and vasa deferentia of the domestic cat","volume":"47","author":"Hay MA","year":"1993","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12142"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01432.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)91849-6"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.05.003"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00397-6"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00571-4"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.11.005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)00066-6"},{"key":"e_1_2_8_20_1","first-page":"85","article-title":"Untersuchung zur Gefrier\u2010Konservierung von Ebersperma. 1. 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