{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:26:05Z","timestamp":1740126365007,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T00:00:00Z","timestamp":1423785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Portuguese Science and Technology Foundation (FCT)","award":["PEst-OE\/AGR\/UI0772\/2011 and 2014"],"award-info":[{"award-number":["PEst-OE\/AGR\/UI0772\/2011 and 2014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Cyclooxygenase\u20102 (<jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102) is overexpressed in several human and animal neoplasms, including the human endometrial carcinoma. It has been suggested as a prognostic marker and a potential therapeutic target. This study aimed to (i) clarify histological aspects of feline endometrial adenocarcinomas (<jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content>) of the papillary serous type and (ii) characterize <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 immunohistochemical expression in normal, hyperplastic and neoplastic endometrium in this species. Archived paraffin\u2010embedded tissue samples of 33 <jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content>, eight cystic endometrial hyperplasias (<jats:styled-content style=\"fixed-case\">CEH<\/jats:styled-content>s) and 21 samples of normal, healthy endometrium in the follicular (<jats:styled-content style=\"fixed-case\">FS<\/jats:styled-content>; n\u00a0=\u00a010) and luteal (<jats:styled-content style=\"fixed-case\">LS<\/jats:styled-content>; n\u00a0=\u00a011) stages were evaluated. Histological evaluation of haematoxylin and eosin\u2010stained sections of the <jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content> revealed a papillary proliferation of neoplastic cells of serous type, accompanied by clear and multinucleated cells. Other architectural arrangements mainly included solid and tubular growth. Randomly distributed areas of necrosis within the tumours were commonly observed. Invasion of the myometrium, of the serosa and of the vascular and\/or lymphatic vessels was not constant features. The mean number of mitoses was higher in <jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content> compared to non\u2010neoplastic endometrium. <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 scores were lower in <jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content> (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.003) and <jats:styled-content style=\"fixed-case\">CEH<\/jats:styled-content> (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.05) when compared to normal epithelium (<jats:styled-content style=\"fixed-case\">NE<\/jats:styled-content>). The loss of the membrane apical reinforcement in epithelial cells was observed in <jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content> samples, which was accompanied by the dislocation of <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 labelling into the cytoplasm and the perinuclear area; in contrast, in epithelial cells in the healthy and hyperplastic endometria, the immunoreaction showed the characteristic pattern of apical membrane reinforcement, suggestive of the membrane polarization. <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 epithelial scores were higher in the <jats:styled-content style=\"fixed-case\">FS<\/jats:styled-content> than in the <jats:styled-content style=\"fixed-case\">LS<\/jats:styled-content>. No differences were found in stromal <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 expression between normal, <jats:styled-content style=\"fixed-case\">CEH<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FEA<\/jats:styled-content> groups, but it was higher in the <jats:styled-content style=\"fixed-case\">LS<\/jats:styled-content> than in the <jats:styled-content style=\"fixed-case\">FS<\/jats:styled-content>. In summary, loss of <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 compartmentalization in neoplastic epithelial cells might be one of the molecular events underlying endometrial carcinogenesis.<\/jats:p>","DOI":"10.1111\/rda.12497","type":"journal-article","created":{"date-parts":[[2015,2,14]],"date-time":"2015-02-14T04:26:52Z","timestamp":1423888012000},"page":"333-340","source":"Crossref","is-referenced-by-count":11,"title":["Immunohistochemical Expression of Cyclooxygenase\u20102 (<scp>COX<\/scp>\u20102) in Feline Endometrial Adenocarcinoma and in Normal and Hyperplastic Endometria"],"prefix":"10.1111","volume":"50","author":[{"given":"AL","family":"Saraiva","sequence":"first","affiliation":[{"name":"CECAV Centro de Ci\u00eancia Animal e Veterin\u00e1ria Universidade de Tr\u00e1s\u2010os\u2010Montes e Alto Douro Quinta de Prados Vila Real Portugal"},{"name":"Escola Universit\u00e1ria Vasco da Gama (EUVG) Coimbra 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