{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:33:22Z","timestamp":1761896002255},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,21]],"date-time":"2015-02-21T00:00:00Z","timestamp":1424476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Contents<\/jats:title>The development of pyometra in cows depends largely on the state of local immunity of the uterus. The objective of the study was to evaluate the concentration of the following proinflammatory cytokines: tumour necrosis factor (TNF<\/jats:styled-content>\u2010\u03b1<\/jats:italic>) and interleukin\u20106 (IL<\/jats:styled-content>\u20106); anti\u2010inflammatory cytokine: interleukin\u201010 (IL<\/jats:styled-content>\u201010); and acute\u2010phase proteins (APP<\/jats:styled-content>s): haptoglobin (H<\/jats:styled-content>p) and serum amyloid A<\/jats:styled-content> (SAA<\/jats:styled-content>), in serum and uterine washings in cows with pyometra and healthy animals. The study was performed on 20 cows divided into two groups based on the results of cytological and ultrasonographic tests: a pyometra and a healthy group (10 cows per group). Experimental material consisted of blood serum and uterine washings. The levels of the following cytokines, TNF<\/jats:styled-content>\u2010\u03b1<\/jats:italic>, IL<\/jats:styled-content>\u20106, IL<\/jats:styled-content>\u201010 and APP<\/jats:styled-content>s \u2013 H<\/jats:styled-content>p and SAA<\/jats:styled-content>, in the study material were determined by ELISA<\/jats:styled-content>. The results showed that the values of TNF<\/jats:styled-content>\u2010\u03b1<\/jats:italic>, IL<\/jats:styled-content>\u20106, IL<\/jats:styled-content>\u201010 as well as SAA<\/jats:styled-content> and H<\/jats:styled-content>p were significantly higher in serum of cows with pyometra compared to controls (p\u00a0<\u00a00.001). The uterine washings had significantly higher levels of IL<\/jats:styled-content>\u20106, IL<\/jats:styled-content>\u201010, and H<\/jats:styled-content>p in pyometra cows compared to the control (p\u00a0<\u00a00.001). Our results indicate that it is possible to monitor the course of pyometra in cows based on the evaluation of the concentration of cytokines and H<\/jats:styled-content>p in the serum and uterine washings. Simultaneous evaluation of selected indicators of antagonistic interaction can be helpful in determining the current status of local immunity of the uterus. On this basis, it could be possible to properly select an adjunctive therapy in the form of immunomodulating preparations.<\/jats:p>","DOI":"10.1111\/rda.12507","type":"journal-article","created":{"date-parts":[[2015,2,21]],"date-time":"2015-02-21T07:08:55Z","timestamp":1424502535000},"page":"417-422","source":"Crossref","is-referenced-by-count":13,"title":["The Concentrations of Inflammatory Cytokines and Acute\u2010Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with Pyometra"],"prefix":"10.1111","volume":"50","author":[{"given":"P","family":"Brodzki","sequence":"first","affiliation":[{"name":"Department and Clinic of Reproduction Faculty of Veterinary Medicine University of Life Sciences in Lublin Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Kostro","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Infectious Diseases Faculty of Veterinary Medicine University of Life Sciences 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