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This study evaluated the relationship between sperm concentration and packaging on <jats:italic>in\u00a0vitro<\/jats:italic> quality of chilled rabbit semen over 96\u00a0h. Semen was collected from adult bucks (n\u00a0=\u00a04) and pooled at 37\u00b0C following evaluation. Pooled ejaculates were diluted with a Tris\u2010based extender supplemented with 100\u00a0\u03bc<jats:sc>m<\/jats:sc> quercetin to a concentration of 15, 30 or 60\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0spermatozoa\/ml, packaged into plastic tubes or 0.5\u2010ml straws and stored at 15\u00b0C. Sperm quality was assessed by computer\u2010assisted sperm Analysis [total motility (<jats:styled-content style=\"fixed-case\">tMOT<\/jats:styled-content>)] and flow cytometry [viability, acrosome integrity, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production, plasma membrane disorder, apoptosis and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation index (<jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content>)] at 0, 48, 72 and 96\u00a0h. From 48\u00a0h, concentrations of 30 and 60\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0spermatozoa\/ml reported the highest <jats:styled-content style=\"fixed-case\">tMOT<\/jats:styled-content>, irrespective of storage vessel (p\u00a0&lt;\u00a00.05). Storage in straws reduced oxidative stress and improved plasma membrane stability. The %<jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content>, mean <jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content> were increased in spermatozoa stored in tubes compared with straws (p\u00a0&lt;\u00a00.05). Although the use of low sperm concentrations in artificial insemination doses would facilitate greater dispersion of genetically superior rabbit bucks, dilution to 15\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0spermatozoa\/ml had a detrimental impact on motility. As such, chilled storage at 30\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0spermatozoa\/ml may provide a suitable balance between motility and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production to best maintain overall sperm function and should be evaluated in a large\u2010scale AI trial.<\/jats:p>","DOI":"10.1111\/rda.12525","type":"journal-article","created":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T12:03:08Z","timestamp":1430740988000},"page":"567-573","source":"Crossref","is-referenced-by-count":13,"title":["The Effect of Sperm Concentration and Storage Vessel on Quercetin\u2010Supplemented Rabbit Semen During Chilled Storage"],"prefix":"10.1111","volume":"50","author":[{"given":"D","family":"Johinke","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science University of Sydney Camperdown NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SP","family":"de Graaf","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science University of Sydney Camperdown NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Bathgate","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science University of Sydney Camperdown NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19991101)284:6<696::AID-JEZ11>3.0.CO;2-E"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950659"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.026"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.07.008"},{"key":"e_1_2_9_6_1","first-page":"71","article-title":"Guidelines for the handling of rabbit bucks and semen","volume":"13","author":"Boiti C","year":"2005","journal-title":"World Rabbit Sci"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00197-X"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2006.12.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(99)00051-2"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00181-0"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.06.010"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/cpt.2005.3.165"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.108.006254"},{"volume-title":"Salamon's Artificial Insemination of Sheep and Goats","year":"1987","author":"Evans G","key":"e_1_2_9_14_1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009844109023"},{"volume-title":"Sperm Chromatin Structure Assay for Fertility Assessment. 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