{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T09:12:19Z","timestamp":1781946739157,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,5,15]],"date-time":"2015-05-15T00:00:00Z","timestamp":1431648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Aquaporins (<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s) are essential membrane protein channels for the transport of water across membranes. Fluid movement in the epididymis is important for modulation of the luminal environment, in which sperm mature and reside. This study was designed to understand the morphology and localization of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s in ram efferent ducts (<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>) and epididymis. For this purpose, the epididymis of seven animals were removed for histologic and immunohistochemical analyses. <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1 immunoreactivity was observed in the apex of the <jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 was found adjacent to the nuclei of the epithelial cells of the <jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>. The epithelial lining of ram epididymis is pseudostratified columnar and presents principal, basal, apical and narrow cells. In the initial segment (<jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content>), a moderate reaction for <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1 was observed in the apical cytoplasm of epithelial cells. An intense reactivity for <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1 was noted over the microvilli of principal cells and in spermatozoa in the caput. In the corpus and cauda, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1 was noted only over the endothelial cells of vascular channels located in intertubular spaces. A weak\u2010to\u2010moderate reaction for <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 was observed in the nuclei of epithelial cells in the <jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content>, caput and corpus of the epididymis. In the cauda, an intense reaction to <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 was observed in the epithelial border. In the <jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content>, caput and corpus, the reactivity for <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9 differed from those observed in domestic animals. The cauda showed a pattern similar to that previously described. These results indicate that <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s 1 and 9 have reversed locations and roles in rams, suggesting activity variations related with fluid and solute absorption throughout the epididymis.<\/jats:p>","DOI":"10.1111\/rda.12537","type":"journal-article","created":{"date-parts":[[2015,5,15]],"date-time":"2015-05-15T11:33:51Z","timestamp":1431689631000},"page":"617-624","source":"Crossref","is-referenced-by-count":17,"title":["Immunolocalization of Aquaporins 1 and 9 in the Ram Efferent Ducts and Epididymis"],"prefix":"10.1111","volume":"50","author":[{"given":"BC","family":"Schimming","sequence":"first","affiliation":[{"name":"Department of Anatomy Biosciences Institute of Botucatu UNESP \u2013 Universidade Estadual Paulista Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PFF","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Department of Anatomy Biosciences 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