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Objective single ejaculate evaluation and individual boar fertility prediction may be possible by introducing automated flow cytometric semen analysis with vital stains (e.g. acrosomal integrity and mito\u2010potential),<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>fragmentation analysis and biomarkers (ubiquitin,<jats:styled-content style=\"fixed-case\">PAWP<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">ALOX<\/jats:styled-content>15, aggresome) associated with normal or defective sperm phenotypes. Measurement of sperm\u2010produced reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) is a helpful indicator of normal semen sample. Semen<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>levels could be managed by the addition of<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\u2010scavenging antioxidants. Alternative energy regeneration substrates and sperm stimulants such as inorganic pyrophosphate and caffeine could increase sperm lifespan in extended semen and within the female reproductive system. Such technology could be combined with timed sperm release in the female reproductive system after artificial insemination. Sperm phenotype analysis by the image\u2010based flow cytometry will go hand in hand with the advancement of swine genomics, linking aberrant sperm phenotype to the fertility influencing gene polymorphisms. Finally, poor\u2010quality ejaculates could be rescued and acceptable ejaculates improved by semen purification methods such as the nanoparticle\u2010based semen purification and magnetic\u2010activated sperm sorting. 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