{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:13:29Z","timestamp":1791562409007,"version":"4.3.4"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T00:00:00Z","timestamp":1445472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2010\/52298\u20105"],"award-info":[{"award-number":["2010\/52298\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["134668\/2012\u20100"],"award-info":[{"award-number":["134668\/2012\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>This study evaluated the performance of an immunochromatographic test (<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>) for the diagnosis of canine brucellosis caused by<jats:italic>Brucella canis<\/jats:italic>, comparing its results with that of the rapid slide agglutination test with and without the use of 2\u2010mercaptoethanol and the agar gel immunodiffusion test (<jats:styled-content style=\"fixed-case\">AGID<\/jats:styled-content>). The microbiological culture,<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>in blood, semen and vaginal swab, a total of 102 dogs were divided into three groups:<jats:italic>B.\u00a0canis<\/jats:italic>\u2010infected dogs (Group 1),<jats:italic>B. canis<\/jats:italic>\u2010non\u2010infected dogs (Group 2) and dogs with suspected brucellosis (Group 3). The diagnostic sensitivity of<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>, 2<jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">AGID<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>in Group 1 was, respectively, 75%, 37.5%, 27.8% and 89.58%. The diagnostic specificity of<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>, 2<jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">AGID<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>in Group 2 was, respectively, 91%, 100%, 100%, and 100%. In dogs with suspected brucellosis, 9.67% were<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>positive, none was positive by 2<jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>, 3.22% were<jats:styled-content style=\"fixed-case\">AGID<\/jats:styled-content>positive and 6.45% were<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>positive. The main drawback concerning canine brucellosis diagnosis is the lack of a highly sensitive serological assay to be used as a screening test to the rapid identification of infected animals. The<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>showed a high diagnostic specificity and a diagnostic sensitivity value greater than that observed in the<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>, 2<jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RSAT<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">AGID<\/jats:styled-content>. However, 10.41% of infected dogs had negative results by<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>. These dogs were positive by microbiological culture and\/or<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, indicating active infection and consequently a higher potential of spreading<jats:italic>Brucella<\/jats:italic>. Although rapid and simple to perform, the<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>lacked sensitivity to be used as a screening test.<\/jats:p>","DOI":"10.1111\/rda.12612","type":"journal-article","created":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T10:16:17Z","timestamp":1445508977000},"page":"939-944","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of an Immunochromatographic Test to the Diagnosis of Canine Brucellosis Caused by<i>Brucella canis<\/i>"],"prefix":"10.1111","volume":"50","author":[{"given":"LB","family":"Keid","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JA","family":"Diniz","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TMFS","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HL","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RM","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,22]]},"reference":[{"key":"e_1_2_5_2_1","volume-title":"Las Tecnicas de Laboratorios en la Brucelosis","author":"Alton GG","year":"1976"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/jcm.15.2.286-289.1982","article-title":"Improved rapid slide agglutination test for presumptive diagnosis of canine brucellosis","volume":"15","author":"Badakhsh FF","year":"1982","journal-title":"Clin Microbiol"},{"key":"e_1_2_5_4_1","first-page":"265","article-title":"Selective media for the isolation of Brucella ovis","volume":"61","author":"Brown GM","year":"1971","journal-title":"Cornell Vet"},{"key":"e_1_2_5_5_1","first-page":"335","volume-title":"Animal Brucellosis","author":"Carmichael LE","year":"1990"},{"key":"e_1_2_5_6_1","first-page":"76","article-title":"Canine brucellosis. 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